This study focuses on analyzing the protein expression pattern of intracellular proteins when Pseudomonas mendocina SMSKVR-3 exposed to 300 mM of arsenate to find out the proteins that are overexpressed or exclusively expressed in response to arsenate. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of protein expression at different time intervals showed the highest number of protein bands (14) that are overexpressed at 8 h of the time interval. It was also observed that treatment with at least 200 mM of As(V) is required to induce a difference in protein expression. Two-dimensional (2D)-PAGE analysis of 8-h sample exhibited 146 unique spots, 45 underexpressed, and 46 overexpressed spots in arsenate-treated sample. Based on the highest percent volume and fold change, three unique spots and one overexpressed spot were selected and analyzed by matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF/TOF) mass spectrometry (MS) analysis followed by the MASCOT search. These proteins were identified as ribosome-recycling factor (20.13 kDa), polyphosphate:ADP/GDP phosphotransferase (40.88 kDa), ribonuclease P protein component (14.96 kDa) and cobalt-precorrin-5B C(1)-methyltransferase (38.43 kDa) with MASCOT score of 54, 81, 94, and 100, respectively. All of these proteins help the bacteria to overcome arsenate stress.

中文翻译：

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砷酸胁迫下门多西假单胞菌SMSKVR-3蛋白的差异表达

本研究的重点是分析门多克假单胞菌 SMSKVR-3 暴露于 300 mM 砷酸盐时细胞内蛋白质的蛋白质表达模式，以找出响应砷酸盐而过度表达或专门表达的蛋白质。不同时间间隔的蛋白质表达的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分析显示，在时间间隔的 8 小时内过表达的蛋白质条带数量最多 (14)。还观察到需要用至少 200 mM As(V) 处理才能诱导蛋白质表达的差异。8 小时样品的二维 (2D)-PAGE 分析在砷酸盐处理的样品中显示 146 个独特点、45 个低表达和 46 个过表达点。基于最高百分比的体积和折叠变化，通过基质辅助激光解吸/电离飞行时间 (MALDI-TOF/TOF) 质谱 (MS) 分析和 MASCOT 搜索选择并分析了三个独特的点和一个过表达的点。这些蛋白质被鉴定为核糖体再循环因子 (20.13 kDa)、多磷酸盐：ADP/GDP 磷酸转移酶 (40.88 kDa)、核糖核酸酶 P 蛋白组分 (14.96 kDa) 和钴前体-5B C(1)-甲基转移酶 (38.43 kDa) MASCOT 得分分别为 54、81、94 和 100。所有这些蛋白质都有助于细菌克服砷酸盐压力。核糖核酸酶 P 蛋白组分 (14.96 kDa) 和钴前体-5B C(1)-甲基转移酶 (38.43 kDa)，MASCOT 评分分别为 54、81、94 和 100。所有这些蛋白质都有助于细菌克服砷酸盐压力。核糖核酸酶 P 蛋白组分 (14.96 kDa) 和钴前体-5B C(1)-甲基转移酶 (38.43 kDa)，MASCOT 评分分别为 54、81、94 和 100。所有这些蛋白质都有助于细菌克服砷酸盐压力。