AIMS Spinal cord injury (SCI) can cause a variety of cells apoptosis, neurodegeneration, and eventually permanent paralysis. This study aimed to examine whether transplanting human umbilical cord mesenchymal stem cells (hucMSCs) can promote locomotor function recovery, reduce apoptosis and inhibit demyelination in SCI models. MAIN METHODS Rats were allocated into Sham group (spinal cord exposure only), SCI + PBS group (spinal cord impact plus phosphate-buffered saline (PBS) injections), SCI + hucMSCs group (spinal cord impact plus hucMSCs injections) groups. Behavioral tests, Basso-Beattie-Bresnahan locomotion scores (BBB scores), were carried out at 0, 3, 7, 14, 21, 28 days after SCI surgery. Hematoxylin-eosin staining observed spinal cord morphology. Nissl staining detected the number of nissl bodies. Myelin basic protein (MBP) and oligodendrocyte (CNPase) were examed by immunohistochemical staining. The apoptosis of oligodendrocyte and neurons were detected by immunofluorescence. RESULTS The 28-day behavioral test showed that the BBB score of rats in the SCI + hucMSCs group increased significantly, comparing to the SCI + PBS group. The numbers of nissl bodies and myelin sheath in the damaged area of SCI + hucMSCs group were also significantly increased compared to the SCI + PBS group. HucMSCs transplanting decreased the expression of protein level of Caspase-3 and Bax and increased the Bcl-2, MBP and CNPase, rescued the apoptosis of neurons and the oligodendrocyte. CONCLUSION These results showed that hucMSCs can improve motor function, tissue repairing and reducing apoptosis in SCI rats.

中文翻译：

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HucMSCs移植促进大鼠SCI后运动功能恢复，减少细胞凋亡并抑制脱髓鞘

AIMS 脊髓损伤 (SCI) 可导致多种细胞凋亡、神经变性，并最终导致永久性瘫痪。本研究旨在研究移植人脐带间充质干细胞（hucMSCs）是否能促进SCI模型中运动功能恢复、减少细胞凋亡和抑制脱髓鞘。主要方法将大鼠分为Sham组（仅脊髓暴露）、SCI+PBS组（脊髓撞击加磷酸盐缓冲盐水（PBS）注射）、SCI+hucMSCs组（脊髓撞击加hucMSCs注射）组。在 SCI 手术后 0、3、7、14、21、28 天进行行为测试，即 Basso-Beattie-Bresnahan 运动评分（BBB 评分）。苏木精-伊红染色观察脊髓形态。尼氏染色检测尼氏体的数量。通过免疫组织化学染色检查髓鞘碱性蛋白（MBP）和少突胶质细胞（CNPase）。免疫荧光法检测少突胶质细胞和神经元凋亡。结果 28天行为测试显示SCI+hucMSCs组大鼠BBB评分较SCI+PBS组显着升高。SCI+hucMSCs组损伤区域的nissl体和髓鞘数量也较SCI+PBS组显着增加。HucMSCs移植降低了Caspase-3和Bax蛋白的表达，增加了Bcl-2、MBP和CNPase，挽救了神经元和少突胶质细胞的凋亡。结论这些结果表明hucMSCs可以改善SCI大鼠的运动功能、组织修复和减少细胞凋亡。免疫荧光法检测少突胶质细胞和神经元凋亡。结果 28天行为测试显示SCI+hucMSCs组大鼠BBB评分较SCI+PBS组显着升高。SCI+hucMSCs组损伤区域的nissl体和髓鞘数量也较SCI+PBS组显着增加。HucMSCs移植降低了Caspase-3和Bax蛋白的表达，增加了Bcl-2、MBP和CNPase，挽救了神经元和少突胶质细胞的凋亡。结论这些结果表明hucMSCs可以改善SCI大鼠的运动功能、组织修复和减少细胞凋亡。免疫荧光法检测少突胶质细胞和神经元凋亡。结果 28天行为测试显示SCI+hucMSCs组大鼠BBB评分较SCI+PBS组显着升高。SCI+hucMSCs组损伤区域的nissl体和髓鞘数量也较SCI+PBS组显着增加。HucMSCs移植降低了Caspase-3和Bax蛋白的表达，增加了Bcl-2、MBP和CNPase，挽救了神经元和少突胶质细胞的凋亡。结论这些结果表明hucMSCs可以改善SCI大鼠的运动功能、组织修复和减少细胞凋亡。结果 28天行为测试显示SCI+hucMSCs组大鼠BBB评分较SCI+PBS组显着升高。SCI+hucMSCs组损伤区域的nissl体和髓鞘数量也较SCI+PBS组显着增加。HucMSCs移植降低了Caspase-3和Bax蛋白的表达，增加了Bcl-2、MBP和CNPase，挽救了神经元和少突胶质细胞的凋亡。结论这些结果表明hucMSCs可以改善SCI大鼠的运动功能、组织修复和减少细胞凋亡。结果 28天行为测试显示SCI+hucMSCs组大鼠BBB评分较SCI+PBS组显着升高。SCI+hucMSCs组损伤区域的nissl体和髓鞘数量也较SCI+PBS组显着增加。HucMSCs移植降低了Caspase-3和Bax蛋白的表达，增加了Bcl-2、MBP和CNPase，挽救了神经元和少突胶质细胞的凋亡。结论这些结果表明hucMSCs可以改善SCI大鼠的运动功能、组织修复和减少细胞凋亡。HucMSCs移植降低了Caspase-3和Bax蛋白的表达，增加了Bcl-2、MBP和CNPase，挽救了神经元和少突胶质细胞的凋亡。结论这些结果表明hucMSCs可以改善SCI大鼠的运动功能、组织修复和减少细胞凋亡。HucMSCs移植降低了Caspase-3和Bax蛋白的表达，增加了Bcl-2、MBP和CNPase，挽救了神经元和少突胶质细胞的凋亡。结论这些结果表明hucMSCs可以改善SCI大鼠的运动功能、组织修复和减少细胞凋亡。