Rubusoside is a natural botanic sweetener with capacity of micelle solubilization. This study revealed the reaction pathway of enzymatically producing rubusoside from a high concentration of stevioside in the absence or presence of rebaudioside A, the identification of the byproduct, as well as the performance of rubusoside in micelle solublization of vitamins. Rebaudioside A initially stimulated rubusoside yield by taking glucosyl from stevioside to form monoglucosyl rebaudioside A, although the β-glucosidase administered was absolutely specific towards stevioside. A trace byproduct was identified as steviol glucosyl ester, yielded from breaking the 13-(β-D-glucopyranosyloxy) linkage of stevioside instead of that of rubusoside. 1% Rubusoside promoted the solubility of vitamin E (VE) and vitamin D₂ at 25℃ by 10⁵ and 111 times, respectively. The driving force for the solubilizing is the synergic effect of VE and rubusoside on the surface activity of rubusoside solution, presented as the erasing of the γ-lgC plateau by VE with increasing concentration, which dramatically increased the concentration for micelle formation. The results are beneficial for the use of concentrated stevia (Stevia rebaudiana Bertoni) extracts to produce rare natural steviol glycoside, yet extend its application in addition to its function as a sweetener.

红景天苷是具有胶束增溶能力的天然植物甜味剂。这项研究揭示了在不存在或存在莱鲍迪甙A的情况下，从高浓度甜菊糖苷中酶法生产出的红景天苷的反应途径，副产物的鉴定以及红景天苷在胶束增溶维生素中的性能。莱鲍迪甙A最初通过从甜菊糖苷中获取葡萄糖基来形成单葡糖基莱鲍迪苷A，从而刺激了卢比索甙的产量，尽管所用的β-葡萄糖苷酶对甜菊糖苷具有绝对的特异性。鉴定出痕量副产物为甜菊醇葡糖基酯，是通过破坏13-（β-D-葡萄糖吡喃糖基氧基）取代了甜菊糖苷。1％的红景天苷在25℃时维生素E（VE）和维生素D ₂的溶解度分别提高了10 ⁵和111倍。增溶的驱动力是VE和红景天苷对红景天苷溶液表面活性的协同作用，表现为VE随着浓度的增加而擦除γ-lgC平台，这显着增加了胶束形成的浓度。该结果有利于浓缩甜叶菊（甜叶菊甜叶菊）提取物用于生产稀有天然甜菊醇糖苷的用途，但除了其作为甜味剂的功能外，还扩大了其应用范围。