The inoculation response of single arbuscular mycorrhiza fungi (AMF) or rhizobia (Rh) in relation to nitrogen (N) acquisition of plants is well established, while the combined effect of both AMF and Rh is poorly known with regard to N assimilation for changes in amino acids of white clover (Trifolium repens). A pot study was carried out to evaluate the effect of single versus dual inoculation of AMF (Rhizophagus intraradices) and rhizobium (Rhizobium trifolii) on plant growth, leaf and root N contents, root amino acid contents, and root N-related enzyme activities in white clover. One hundred days after inoculations, Rh inoculation significantly stimulated the root colonization by R. intraradices. A single inoculation of AMF or Rh improved the plant growth (biomass production, root projected area, and root volume), root N acquisition, and dual inoculation of AMF and Rh further expanded some of these positive effects on root projected area and root N contents than single inoculation. All the inoculations notably increased activities of root asparagines synthase, nitrate reductase, and glutamate synthase, whilst dual inoculation displayed a much stronger effect in asparagine synthase activity than single inoculation. Single Rh treatment increased root glutamate and proline content, single AMF inoculation induced an increase in glutamate, aspartate, arginine, and ornithine content, while dual inoculation stimulated the accumulation of aspartate and proline. These results suggested the cooperation between the AMF and Rh inoculations, which magnified the positive effect on partly N metabolites and N-assimilation relevant enzymes of white clover.

相对于植物获得氮（N）而言，单株丛枝菌根真菌（AMF）或根瘤菌（Rh）的接种反应已得到充分确立，而对于N的同化变化，AMF和Rh的联合作用却鲜为人知。白三叶草的氨基酸（三叶草）。甲锅进行了研究，以评估单相对于AMF（双接种效果Rhizophagus intraradices）和根瘤菌（根瘤菌三叶草对植物的生长，叶和根N含量，根氨基酸含量），并且在根的N-相关酶活性白三叶草。接种一百天后，Rh接种显着刺激了R的根定植。内半径。单次接种AMF或Rh可以改善植物的生长（生物量生产，根投影面积和根部体积），根系氮素的获得，而AMF和Rh双重接种可以进一步扩大对根部投影面积和根系N含量的一些积极影响。比单次接种。所有接种均显着提高了根天冬酰胺合成酶，硝酸盐还原酶和谷氨酸合酶的活性，而双重接种显示出比单次接种强得多的天冬酰胺合成酶活性。单次Rh处理可增加根部谷氨酸和脯氨酸的含量，单次AMF接种可引起谷氨酸，天冬氨酸，精氨酸和鸟氨酸含量的增加，而双重接种可刺激天冬氨酸和脯氨酸的积累。这些结果表明AMF和Rh接种之间的合作，