Abstract

Consolidated bioprocessing (CBP) featuring concomitant hydrolysis of renewable substrates and microbial conversion into value-added biomolecules is considered to bring substantial benefits to the overall process efficiency. The biggest challenge in developing an economically feasible CBP process is identification of bifunctional biocatalyst merging the ability to utilize the substrate and convert it to value-added product with high efficiency. Yarrowia lipolytica is known for its exceptional performance in hydrophobic substrates assimilation and storage. On the other hand, its capacity to grow on plant-derived biomass is strongly limited. Still, its high potential to simultaneously overproduce several secretory proteins makes Y. lipolytica a platform of choice for expanding its substrate range to complex polysaccharides by engineering its hydrolytic secretome. This review provides an overview of different genetic engineering strategies advancing development of Y. lipolytica strains able to grow on the following four complex polysaccharides: starch, cellulose, xylan, and inulin. Much attention has been paid to genome mining studies uncovering native potential of this species to assimilate untypical sugars, as in many cases it turns out that dormant pathways are present in Y. lipolytica’s genome. In addition, the magnitude of the economic gain by CBP processing is here discussed and supported with adequate calculations based on simulated process models.

Key points

• The mini-review updates the knowledge on polysaccharide-utilizing Yarrowia lipolytica.

• Insight into molecular bases founding new biochemical qualities is provided.

• Model industrial processes were simulated and the associated costs were calculated.

中文翻译：

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解脂耶氏酵母的水解分泌组工程技术，用于多糖资源的整合生物加工：淀粉，纤维素，木聚糖和菊粉综述

摘要

合并生物处理（CBP）具有可再生底物伴随水解和微生物转化成增值生物分子的功能，被认为可为整体处理效率带来实质性好处。开发经济可行的CBP工艺的最大挑战是鉴定双功能生物催化剂，以兼顾利用底物并将其高效转化为增值产品的能力。解脂耶氏酵母（Yarrowia lipolytica）以其在疏水性底物同化和储存中的卓越性能而闻名。另一方面，其在植物衍生的生物质上生长的能力受到极大限制。尽管如此，它具有同时过量生产几种分泌蛋白的巨大潜力，从而使解脂耶氏酵母通过工程化其水解分泌组将其底物范围扩展至复杂多糖的理想平台。这篇综述概述了促进解脂耶氏酵母菌株发展的不同基因工程策略，这些菌株能够在以下四种复杂多糖上生长：淀粉，纤维素，木聚糖和菊粉。人们已经对基因组挖掘研究给予了很多关注，这些研究揭示了该物种吸收非典型糖的天然潜力，因为在许多情况下，事实证明解脂耶氏酵母的基因组中存在休眠途径。另外，在此讨论了通过CBP处理获得的经济收益的幅度，并在基于模拟过程模型的基础上进行了适当的计算，以提供支持。

关键点

•迷你评论更新了利用多糖的解脂耶氏酵母的知识。

•提供对分子基础的洞察力，以发现新的生化特性。

•模拟了模型工业流程并计算了相关成本。