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Selenium-Containing Amino Acids Protect Dextran Sulfate Sodium-Induced Colitis via Ameliorating Oxidative Stress and Intestinal Inflammation
Journal of Inflammation Research ( IF 4.5 ) Pub Date : 2021-01-14 , DOI: 10.2147/jir.s288412
Chengxin Shi 1 , Fengli Yue 2 , Feiyu Shi 1 , Qian Qin 1 , Lizhao Wang 1 , Guanghui Wang 1 , Lijun Mu 1 , Dan Liu 1 , Yaguang Li 1 , Tianyu Yu 1 , Junjun She 1
Affiliation  

Background: Inflammatory bowel disease (IBD) is characterized by chronic relapsing inflammation of the gastrointestinal tract. Oxidative stress plays a pivotal role in the pathogenesis of IBD. Selenium-containing amino acids reportedly have anti-oxidative and anti-inflammatory properties, but it remains unknown if selenium-containing amino acids can be used to treat IBD. This study aimed to investigate the effects of two selenium-containing amino acids – selenocysteine and selenocystine – on oxidative stress and chronic inflammation in a mouse model of dextran sulfate sodium (DSS)-induced IBD.
Methodology: C57BL/6 mice were randomly assigned to the following six groups: control, DSS, DSS+selenocysteine, DSS+selenocystine, DSS+sodium selenite, and DSS+N-acetylcysteine (NAC). IBD was induced by 3% DSS. Pro-inflammatory cytokines [interleukin-1β (IL-1β), monocyte chemotactic protein 1 (MCP-1), IL-6, and tumor necrosis factor-α (TNF-α)] and markers for oxidative and anti-oxidative stress [malondialdehyde (MDA), reactive oxygen species (ROS), superoxide dismutase (SOD), and glutathione peroxidase (GPx)] were measured using immunohistochemical analysis.
Results: Selenocysteine and selenocystine significantly attenuated IBD-related symptoms, including preventing weight loss, decreasing disease activity index (DAI) scores, and increasing colon length. Selenocysteine and selenocystine significantly ameliorated the DSS-induced oxidative stress, as demonstrated by a reduction in ROS and MDA activity and an increase in SOD and GPx activity. IL-1, MCP-1, IL-6, and TNF-α levels were significantly increased in the IBD mice, while treatment with the selenium-containing amino acids significantly reduced the levels of these pro-inflammatory cytokines. In vivo safety analysis showed minimal side effects of the selenium-containing amino acids.
Conclusion: We found that selenocysteine and selenocystine ameliorated DSS-induced IBD via reducing oxidative stress and intestinal inflammation, indicating that selenium-containing amino acids could be a novel therapeutic option for patients with IBD.



中文翻译:

含硒氨基酸通过改善氧化应激和肠道炎症来保护葡聚糖硫酸钠诱导的结肠炎

背景:炎症性肠病(IBD)的特征是胃肠道的慢性复发性炎症。氧化应激在 IBD 的发病机制中起关键作用。据报道,含硒氨基酸具有抗氧化和抗炎特性,但含硒氨基酸是否可用于治疗 IBD 尚不清楚。本研究旨在研究两种含硒氨基酸——硒代半胱氨酸和硒代半胱氨酸——对葡聚糖硫酸钠 (DSS) 诱导的 IBD 小鼠模型中氧化应激和慢性炎症的影响。
方法:C57BL/6 小鼠被随机分配到以下六组:对照组、DSS、DSS+硒代半胱氨酸、DSS+硒代半胱氨酸、DSS+亚硒酸钠和DSS+N-乙酰半胱氨酸(NAC)。IBD 由 3% DSS 诱导。促炎细胞因子 [白细胞介素-1β (IL-1β)、单核细胞趋化蛋白 1 (MCP-1)、IL-6 和肿瘤坏死因子-α (TNF-α)] 以及氧化和抗氧化应激的标志物 [使用免疫组织化学分析测量丙二醛 (MDA)、活性氧 (ROS)、超氧化物歧化酶 (SOD) 和谷胱甘肽过氧化物酶 (GPx)]。
结果:硒代半胱氨酸和硒代半胱氨酸显着减轻 IBD 相关症状,包括防止体重减轻、降低疾病活动指数 (DAI) 评分和增加结肠长度。硒代半胱氨酸和硒代半胱氨酸显着改善了 DSS 诱导的氧化应激,表现为 ROS 和 MDA 活性降低以及 SOD 和 GPx 活性增加。IBD 小鼠的 IL-1、MCP-1、IL-6 和 TNF-α 水平显着增加,而含硒氨基酸治疗显着降低了这些促炎细胞因子的水平。体内安全性分析显示含硒氨基酸的副作用最小。
结论:我们发现硒代半胱氨酸和硒代半胱氨酸通过减少氧化应激和肠道炎症来改善 DSS 诱导的 IBD,这表明含硒氨基酸可能是 IBD 患者的一种新的治疗选择。

更新日期:2021-01-14
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