For prevention of the coronavirus disease 2019 caused by the novel coronavirus SARS-CoV-2, an effective vaccine is critical. Herein, several potential peptide epitopes from the spike protein of SARS-CoV-2 have been synthesized and covalently linked with the cross-reactive material (CRM197). Immunization of mice with the resulting conjugates induced high titers of IgG antibodies against the spike protein. Importantly, the post-immune sera effectively neutralized SARS-CoV-2 pseudovirus, suggesting the epitopes identified are protective, and these conjugates are promising leads for anti-SARS-CoV-2 vaccine development.

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基于合成肽的抗 SARS-CoV-2 候选疫苗的合成和免疫学评价

为了预防由新型冠状病毒 SARS-CoV-2 引起的 2019 年冠状病毒病，有效的疫苗至关重要。在此，已经合成了来自 SARS-CoV-2 刺突蛋白的几个潜在肽表位，并与交叉反应材料 (CRM197) 共价连接。用所得缀合物对小鼠进行免疫诱导了针对刺突蛋白的高滴度 IgG 抗体。重要的是，免疫后血清有效地中和了 SARS-CoV-2 假病毒，表明所鉴定的表位具有保护性，这些结合物是抗 SARS-CoV-2 疫苗开发的有希望的线索。