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Proline-rich Extensin-like Receptor Kinases PERK5 and PERK12 are involved in Pollen Tube Growth
bioRxiv - Plant Biology Pub Date : 2021-01-13 , DOI: 10.1101/2021.01.12.425807
Cecilia Borassi , Ana R. Sede , Martin A. Mecchia , Silvina Mangano , Eliana Marzol , Silvina P. Denita-Juarez , Juan D. Salgado Salter , Silvia M. Velasquez , Jorge P. Muschietti , José M. Estevez

Cell wall integrity plays an essential role during cell elongation impacting on polar growth (e.g. in pollen tubes and root hairs). Proline-rich Extensin-like Receptor Kinases (PERK) belong to the hydroxyproline-rich glycoprotein (HRGP) superfamily of cell surface glycoproteins. Here, we identified two PERKs from Arabidopsis thaliana, PERK5 and PERK12 highly expressed in mature pollen and pollen tubes. Pollen tube growth was impaired in the single and double perk5-1 perk12-1 loss of function mutant, with a moderate impact on seed production. Furthermore, double perk5-1 perk12-1 mutants show excessive accumulation of pectins and cellulose at the cell wall pollen of the tube tip as well as an upregulation of cytoplasmic ROS levels. Taken together, our results suggest that PERK5 and PERK12 are necessary for proper pollen tube growth highlighting their role on cell wall assembly and ROS homeostasis.

中文翻译:

富含脯氨酸的类Extensin受体激酶PERK5和PERK12参与花粉管的生长

细胞壁完整性在细胞伸长过程中(对花粉管和根毛)影响极性增长过程中起着至关重要的作用。富含脯氨酸的延伸蛋白样受体激酶(PERK)属于细胞表面糖蛋白的富含羟脯氨酸的糖蛋白(HRGP)超家族。在这里,我们从拟南芥中鉴定了两个PERK,PERK5和PERK12在成熟的花粉和花粉管中高表达。perk5-1 perk12-1功能丧失的单突变体和双突变体中,花粉管的生长受到损害,对种子产量产生中等影响。此外,双perk5-1 perk12-1突变体显示果胶和纤维素在管尖的细胞壁花粉中过度积聚,并上调了细胞质ROS水平。在一起
更新日期:2021-01-14
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