B cells are an adaptive immune target of biomaterials development in vaccine research but despite their role in wound healing have not been studied in tissue engineering and regenerative medicine. We evaluated the B cell response to biomaterial scaffold materials implanted in a muscle wound; a biological extracellular matrix (ECM) and synthetic polyester polycaprolactone. In the local muscle tissue, small numbers of B cells are recruited in response to tissue injury and biomaterial implantation. ECM materials induced plasmablasts in lymph nodes and antigen presentation in the spleen while the synthetic PCL implants delayed B cell migration and induced an antigen presenting phenotype. In muMt- mice lacking B cells, the fibrotic response to the synthetic biomaterials decreased. Immunofluorescence confirmed antigen presenting B cells in fibrotic tissue surrounding silicone breast implants. In sum, the adaptive B cell immune response to biomaterial depends on composition and induces local, regional and systemic immunological changes.

中文翻译：

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生物材料以特定于材料的方式指导功能性B细胞反应

B细胞是疫苗研究中生物材料开发的一种适应性免疫靶标，尽管在组织工程和再生医学中尚未对其B在伤口愈合中的作用进行研究。我们评估了B细胞对植入肌肉伤口的生物材料支架材料的反应。生物细胞外基质（ECM）和合成聚酯聚己内酯。在局部肌肉组织中，响应于组织损伤和生物材料植入，募集了少量的B细胞。ECM材料在淋巴结中诱导浆母细胞和在脾脏中呈递抗原，而合成PCL植入物则延迟B细胞迁移并诱导呈递抗原的表型。在缺少B细胞的muMt-小鼠中，对合成生物材料的纤维化反应降低。免疫荧光法证实了硅胶乳房植入物周围纤维化组织中的抗原呈递B细胞。总之，对生物材料的适应性B细胞免疫反应取决于组成，并引起局部，区域和全身免疫学变化。