Metagenomic next-generation sequencing (mNGS) has been utilized for diagnosing infectious diseases. It is a culture-free and hypothesis-free nucleic acid test for diagnosing all pathogens with known genomic sequences, including bacteria, fungi, viruses and parasites. While this technique greatly expands the clinical capacity of pathogen detection, it is a second-line choice due to lengthy procedures and microbial contaminations introduced from wet-lab processes. As a result, we aimed to reduce the hands-on time and exogenous contaminations in mNGS.

中文翻译：

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临床宏基因组下一代测序自动化解决方案的概念验证研究

宏基因组下一代测序 (mNGS) 已被用于诊断传染病。它是一种无培养和无假设的核酸测试，用于诊断具有已知基因组序列的所有病原体，包括细菌、真菌、病毒和寄生虫。虽然这项技术极大地扩展了病原体检测的临床能力，但由于冗长的程序和湿实验室过程中引入的微生物污染，它是二线选择。因此，我们旨在减少 mNGS 中的手动操作时间和外源性污染。