Based on the untreated biofilm formation in urinary tract infections (UTIs), current study was focused on the inhibition of Pseudomonas aeruginosa (P. aeruginosa) biofilm formation using Nerium oleander (N. oleander) essential oils. Initially, the LC-MS analysis of antibacterial potential of hydro distillated N. oleander essential oils was detected by LC-MS analysis. Initially, the antibacterial nature of essential oils was detected by agar well diffusion method against biofilm forming P. aeruginosa. Then, the possible anti-bacterial fractions of the essential oils were purified by preparative HPLC, and confirmed by analytical HPLC peaks. Possible anti-bacterial components of the fractions were merged together and confirmed by agar well diffusion method. Consecutively, the and minimum biofilm inhibition concentration experiment was proved that the concentration of 200 µg/mL of purified essential oils was very effective against P. aeruginosa biofilm formation. The decrease survival rate of the MTT assay result was also supported the minimum biofilm inhibition concentration result. Finally, all the results were more evident; the HPLC purified N. oleander essential oils as a promising anti-biofilm factor against P. aeruginosa biofilm formation.

基于未治疗的尿路感染（UTIs）生物膜形成，当前的研究重点在于使用夹竹桃夹竹桃（N. oleander）精油抑制铜绿假单胞菌（P. aeruginosa）生物膜形成。最初，通过LC-MS分析检测了水蒸馏的夹竹桃精油的抗菌潜力的LC-MS分析。最初，通过琼脂孔扩散法检测精油对铜绿假单胞菌生物膜形成的抗菌性。。然后，通过制备型HPLC纯化精油可能的抗菌馏分，并通过分析性HPLC峰进行确认。将级分中可能的抗菌成分合并在一起，并通过琼脂井扩散法进行确认。连续地和最小生物膜抑制浓度实验证明，浓度为200 µg / mL的纯化精油对铜绿假单胞菌生物膜形成非常有效。MTT测定结果的降低的存活率也支持最小生物膜抑制浓度的结果。最后，所有结果都更加明显。HPLC纯化的夹竹桃精油作为一种有前景的抗铜绿假单胞菌的生物膜因子 生物膜形成。