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A high-precision thermometry microfluidic chip for real-time monitoring of the physiological process of live tumour cells
Talanta ( IF 6.1 ) Pub Date : 2021-01-14 , DOI: 10.1016/j.talanta.2021.122101
Xuefei Zhao , Wanlei Gao , Jiawen Yin , Weihua Fan , Zhenyu Wang , Kaikai Hu , Yuliang Mai , Anbo Luan , Baojian Xu , Qinghui Jin

Temperature changes in cells are generally accompanied by physiological processes. Cellular temperature measurements can provide important information to fully understand cellular mechanisms. However, temperature measurements with conventional methods, such as fluorescent polymeric thermometers and thermocouples, have limitations of low sensitivity or cell state disturbance. We developed a microfluidic chip integrating a high-precision platinum (Pt) thermo-sensor that can culture cells and monitor the cellular temperature in situ. During detection, a constant temperature system with a stability of 0.015 °C was applied. The temperature coefficient of resistance of the Pt thermo-sensor was 2090 ppm/°C, giving a temperature resolution of the sensor of less than 0.008 °C. This microchip showed a good linear correlation between the temperature and resistance of the Pt sensor at 20–40 °C (R2 = 0.999). Lung and liver cancer cells on the microchip grew normally and continuously. The maximum temperature fluctuation of H1975 (0.924 °C) was larger than that of HepG2 (0.250 °C). However, the temperature of adherent HepG2 cells changed over time, showing susceptibility to the environment most of the time compared to H1975. Moreover, the temperature increment of non-cancerous cells, such as hepatic stellate cells, was monitored in response to the stimulus of paraformaldehyde, showing the process of cell death. Therefore, this thermometric microchip integrated with cell culture could be a non-disposable and label-free tool for monitoring cellular temperature applied to the study of physiology and pathology.



中文翻译:

高精度测温微流控芯片,用于实时监测活肿瘤细胞的生理过程

细胞中的温度变化通常伴随着生理过程。细胞温度测量可以提供重要信息,以全面了解细胞机制。但是,使用常规方法(例如荧光聚合物温度计和热电偶)进行温度测量具有灵敏度低或电池状态干扰的局限性。我们开发了一种集成了高精度铂(Pt)热传感器的微流控芯片,该传感器可以培养细胞并就地监测细胞温度。在检测过程中,使用了稳定度为0.015°C的恒温系统。Pt热传感器的电阻温度系数为2090 ppm /°C,因此传感器的温度分辨率小于0.008°C。2  = 0.999)。微芯片上的肺癌和肝癌细胞正常且连续生长。H1975(0.924°C)的最大温度波动大于HepG2(0.250°C)的最大温度波动。但是,粘附的HepG2细胞的温度会随时间变化,与H1975相比,大多数时间都表现出对环境的敏感性。此外,响应多聚甲醛的刺激,监测非癌性细胞(例如肝星状细胞)的温度升高,显示细胞死亡的过程。因此,这种与细胞培养相结合的测温微芯片可能是一种用于监测细胞温度的非一次性,无标签的工具,可用于生理学和病理学研究。

更新日期:2021-01-22
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