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Panel of suitable reference genes and its gender differences of fetal rat liver under physiological conditions and exposure to dexamethasone during pregnancy
Reproductive Toxicology ( IF 3.3 ) Pub Date : 2021-01-14 , DOI: 10.1016/j.reprotox.2021.01.005
Heze Liu 1 , Liang Liu 2 , Hui Han 2 , Kexin Liu 1 , Hui Wang 1
Affiliation  

The panel of suitable reference genes in the fetal liver have not been reported. In this study, five commonly used reference genes (GAPDH, β-actin, Rn18 s, Rpl13a, and Rps29) were firstly selected as candidates. Bestkeeper, GeNorm, and NormFinder software were then used to screen out the panel of suitable reference genes of male and female fetal rat liver under physiological and prenatal dexamethasone exposure (PDE) conditions. Finally, we verified the reliability of the screened panel of reference genes by standardizing sterol regulatory element binding protein 1c (SREBP1c) expression with different reference genes. The results showed that GAPDH + Rn18 s and GAPDH + Rpl13a were respectively the panel of suitable reference genes in male and female rat fetal liver under the physiological model, while Rn18 s + Rps29 and GAPDH + Rn18 s were respectively under the PDE model. The results showed that different reference genes affected the statistical results of SREBP1c expression, and the screened panel of suitable reference genes under the PDE model had smaller intragroup differences, when compared with other reference genes under physiological and PDE models. In conclusion, we screened and determined that the panel of suitable reference genes were GAPDH + Rn18 s and Rn18 s + Rps29 in the male rat fetal liver under physiological and PDE models, while they were GAPDH + Rpl13a and GAPDH + Rn18 s in the females, and confirmed that the selection of the panel of suitable reference genes in the fetal liver had gender differences and pathological model specificity.



中文翻译:

胎鼠肝脏在生理条件和孕期暴露于地塞米松的适宜参考基因组及其性别差异

胎肝中合适的参考基因组尚未见报道。本研究首先选择了 5 个常用的参考基因(GAPDH、β-actin、Rn18s、Rpl13a 和 Rps29)作为候选基因。然后使用 Bestkeeper、GeNorm 和 NormFinder 软件筛选出在生理和产前地塞米松暴露 (PDE) 条件下雄性和雌性胎鼠肝脏的合适参考基因组。最后,我们通过用不同的参考基因标准化甾醇调节元件结合蛋白 1c (SREBP1c) 的表达来验证筛选的参考基因组的可靠性。结果表明,GAPDH+Rn18s和GAPDH+Rpl13a分别是生理模型下雄性和雌性大鼠胎肝中合适的参考基因组,而 Rn18 s + Rps29 和 GAPDH + Rn18 s 分别在 PDE 模型下。结果表明,不同的参考基因影响了SREBP1c表达的统计结果,与生理和PDE模型下的其他参考基因相比,PDE模型下筛选出的一组合适的参考基因组内差异较小。综上所述,我们筛选并确定了合适的参考基因组在生理和PDE模型下雄性大鼠胎肝中为GAPDH + Rn18 s和Rn18 s + Rps29,而在雌性中为GAPDH + Rpl13a和GAPDH + Rn18 s ,并证实在胎肝中选择合适的参考基因组具有性别差异和病理模型特异性。结果表明,不同的参考基因影响了SREBP1c表达的统计结果,与生理和PDE模型下的其他参考基因相比,PDE模型下筛选出的一组合适的参考基因组内差异较小。综上所述,我们筛选并确定了合适的参考基因组在生理和PDE模型下雄性大鼠胎肝中为GAPDH + Rn18 s和Rn18 s + Rps29,而在雌性中为GAPDH + Rpl13a和GAPDH + Rn18 s ,并证实在胎肝中选择合适的参考基因组具有性别差异和病理模型特异性。结果表明,不同的参考基因影响了SREBP1c表达的统计结果,与生理和PDE模型下的其他参考基因相比,PDE模型下筛选出的一组合适的参考基因组内差异较小。综上所述,我们筛选并确定了合适的参考基因组在生理和PDE模型下雄性大鼠胎肝中为GAPDH + Rn18 s和Rn18 s + Rps29,而在雌性中为GAPDH + Rpl13a和GAPDH + Rn18 s ,并证实在胎肝中选择合适的参考基因组具有性别差异和病理模型特异性。与生理和 PDE 模型下的其他参考基因相比。综上所述,我们筛选并确定了合适的参考基因组在生理和PDE模型下雄性大鼠胎肝中为GAPDH + Rn18 s和Rn18 s + Rps29,而在雌性中为GAPDH + Rpl13a和GAPDH + Rn18 s ,并证实在胎肝中选择合适的参考基因组具有性别差异和病理模型特异性。与生理和 PDE 模型下的其他参考基因相比。综上所述,我们筛选并确定了合适的参考基因组在生理和PDE模型下雄性大鼠胎肝中为GAPDH + Rn18 s和Rn18 s + Rps29,而在雌性中为GAPDH + Rpl13a和GAPDH + Rn18 s ,并证实在胎肝中选择合适的参考基因组具有性别差异和病理模型特异性。

更新日期:2021-01-18
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