The effect of chronic stress on oxidative stress (OS) is commonly discussed while the effect of acute stress situation is not fully examined yet. The present study was aimed to analyse whether acute psychosocial strain causes changes in OS and antioxidant status.

Unstimulated saliva was collected from 46 healthy prepubertal children during the control and stress day. On the stress day, collection was performed before and after a stress situation induced by the Trier social stress test. Saliva collection during the control day imitated the stress day without the stress strain. Samples were used for analysis of lipid peroxidation, thiobarbituric acid reactive substances (TBARS), advanced glycation end products (AGEs) and markers of antioxidant status, total antioxidant capacity (TAC) and ferric reducing antioxidant power (FRAP).

On the stress day, increased level of FRAP was observed in the second saliva collection in comparison with the first collection. Within the same day, no significant changes in the levels of TBARS, AGEs and TAC were observed in samples taken before and after stress strain. Significantly higher levels of TBARS were observed on stress day in comparison to control day.

In summary, acute psychosocial stress caused increase of FRAP during the stress day. TBARS did not increase during the stress day in the second sample but it was higher compared to the control day. None of the interactions with gender were statistically significant. It appears the short-term exposure to stress could potentially stimulate antioxidant activity.

通常讨论慢性应激对氧化应激 (OS) 的影响，而尚未充分研究急性应激情况的影响。本研究旨在分析急性心理社会压力是否会导致 OS 和抗氧化状态的变化。

在对照日和应激日期间，从 46 名青春期前健康儿童收集未受刺激的唾液。在压力日，收集是在特里尔社会压力测试诱发的压力情况之前和之后进行的。对照日期间的唾液收集模仿了没有压力应变的压力日。样品用于分析脂质过氧化、硫代巴比妥酸反应物质 (TBARS)、晚期糖基化终产物 (AGE) 和抗氧化状态标志物、总抗氧化能力 (TAC) 和铁还原抗氧化能力 (FRAP)。

在压力日，与第一次收集相比，在第二次唾液收集中观察到 FRAP 水平增加。在同一天内，在应力应变前后采集的样品中，TBARS、AGEs 和 TAC 的水平没有显着变化。与对照日相比，在压力日观察到显着更高水平的 TBARS。

总之，急性心理社会压力导致压力日期间 FRAP 增加。TBARS 在第二个样品的应激日期间没有增加，但与对照日相比更高。与性别的相互作用均无统计学意义。看来短期承受压力可能会刺激抗氧化活性。