An earlier study showed that hybrid striped bass (Morone chrysops ♀ x M. saxatilis ♂) diets containing 20 % animal meals and up to 30 % aerobically fermented (AF) carinata meal (Brassica carinata, CM) followed by a single wash with water (AFCM) or 30 % CM double-washed with water without AF (WCM), resulted in similar growth. However feed utilization and Fulton’s condition factor of 30 % WCM were better. The current study investigated effects of AFCM and WCM on amino acid digestibilities using naive fish. Effects of AFCM and WCM on trypsin activity, and amino acid availability were determined using hybrid striped bass fed experimental diets for 127 and 148 days, respectively (after a growth trial). High (30 %) AFCM reduced all essential amino acids (EAA) and conditionally EAA (CEAA) except histidine, methionine and threonine. However, all EAA and CEAAs except lysine and cysteine were digested better in 30 %WCM than 30 % AFCM. High (30 %) CMs resulted in generally high serum free methionine and tryptophan, but less leucine for the first 9 h. However, 30 % WCM resulted in generally more serum free arginine and valine than 30 % AFCM. High CMs increased muscle free histidine. Generally, WCM resulted in more muscle lysine and valine than AFCM. Muscle methionine was increased by CMs. Both serum and muscle free total EAAs in hybrid striped bass fed 30 % WCM were elevated continuously over a longer period above the baseline levels. Using EAA:lysine, only muscle histidine were adequate for muscle synthesis over the 36-h period. Overall, amino acids were more available in WCM than AFCM.

早期的研究表明，杂交条纹低音（诸根虻♀X M.鲈♂）含有20％的动物膳食的膳食和高达30％的需氧发酵（AF）芥粉（芥，CM），然后用水进行单次洗涤（AFCM）或用不使用AF的水进行30％CM两次洗涤（WCM），导致相似的生长。但是饲料利用率和富尔顿条件因子为30％WCM更好。目前的研究调查了AFCM和WCM对幼稚鱼氨基酸消化率的影响。AFCM和WCM对胰蛋白酶活性和氨基酸利用率的影响分别使用混合条纹鲈鱼饲喂的实验饮食127天和148天（在生长试验后）确定。高（30％）AFCM会降低所有必需氨基酸（EAA）并有条件地降低EAA（CEAA），除了组氨酸，蛋氨酸和苏氨酸。但是，除赖氨酸和半胱氨酸外，所有EAA和CEAA在30％WCM中的消化效果均好于30％AFCM。高（30％）的CM导致一般的血清游离蛋氨酸和色氨酸水平较高，但前9小时的亮氨酸较少。但是，与30％AFCM相比，30％WCM通常产生更多的无血清精氨酸和缬氨酸。高CM增加肌肉游离组氨酸。通常，WCM比AFCM导致更多的肌肉赖氨酸和缬氨酸。CM可增加肌肉蛋氨酸的含量。饲喂30％WCM的混合条纹鲈鱼的无血清和无肌肉总EAA均在较长时间内持续高于基线水平。使用EAA：赖氨酸，仅肌肉中的组氨酸足以在36小时内进行肌肉合成。总体而言，WCM中的氨基酸比AFCM中的氨基酸更多。饲喂30％WCM的混合条纹鲈鱼的无血清和无肌肉总EAA均在较长时间内持续高于基线水平。使用EAA：赖氨酸，仅肌肉中的组氨酸足以在36小时内进行肌肉合成。总体而言，WCM中的氨基酸比AFCM中的氨基酸更多。饲喂30％WCM的混合条纹鲈鱼的无血清和无肌肉总EAA均在较长时间内持续高于基线水平。使用EAA：赖氨酸，仅肌肉中的组氨酸足以在36小时内进行肌肉合成。总体而言，WCM中的氨基酸比AFCM中的氨基酸更多。