An effective protocol for the plant regeneration via direct and indirect organogenesis has been developed from leaf explants of Asystasia gangetica (L.), cultured on Murashige and Skoog (MS) medium supplemented with various concentrations and combinations of auxin and cytokinins. Approximately 86% of explants produced direct shoots on MS medium containing 0.5 mg L⁻¹ 6-benzyladenine (BA) and 10 μg L⁻¹ Triacontanol (TRIA) with a maximum of 4.82 ± 0.29 shoots per leaf segment. For production of callus-mediated plantlets (indirect), primarily callus was induced on MS medium containing 2 mg L⁻¹ 2,4-dichlorophenoxyacetic acid (2,4-D), which was then subcultured on medium with 0.1 mg L⁻¹ naphthaleneacetic acid (NAA), 0.5 mg L⁻¹ BA, and 1 to 8 mg L⁻¹ 2-isopentenyl adenine (2iP) in order to develop organogenic callus and subsequent shoot induction. A maximum of 6.84 ± 0.05 shoots per callus clump was obtained on MS media supplemented with 4 mg L⁻¹ 2iP, 0.5 mg L⁻¹ BA, and 0.1 mg L⁻¹ NAA. The shootlets produced roots when cultured on half-strength MS media supplemented with 2 mg L⁻¹ indole-3-butyric acid (IBA). In vitro propagated plantlets were hardened on soil rite and acclimatized to field condition with 85% survivability. The chlorophyll content of acclimatized plants was comparable with that of the mother plant, while stomatal micromorphology of regenerated plants exhibited no abnormalities. The radical scavenging and antioxidant activity of methanolic extract of leaves were measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing ability of plasma (FRAP), and phosphomolybdenum test. In all experiments, regenerated plants exhibited enhanced antioxidant potential indicating micropropagated plants could be exploited for isolation of novel biomolecules. Further, the genetic homogeneity of acclimatized plants was confirmed by PCR-based start codon targeted (SCoT) markers and ycf1b DNA barcoding primers which exhibited monomorphic bands identical to the normal mother plant and no variations were observed.

已经通过从Astystasia gangetica（L .）的叶外植体开发了一种通过直接和间接器官发生进行植物再生的有效方案，该植株在Murashige和Skoog（MS）培养基上培养，并补充了各种浓度的生长素和细胞分裂素组合。大约86％的外植体在含有0.5 mg L ^-1 6-苄基腺嘌呤（BA）和10μgL ^-1 Triacontanol（TRIA）的MS培养基上产生直接枝条，每个叶段最多有4.82±0.29个枝条。对于生产的愈伤组织介导的小植株（间接）的，主要是愈伤组织诱导含有2mg L MS培养基上^-1 2,4-二氯苯氧基乙酸（2,4-d），然后将其上培养基中继代培养用0.1毫克的L^-1萘乙酸（NAA），0.5 mg L ^-1 BA和1至8 mg L ^-1 2-异戊烯腺嘌呤（2iP），以发展器官发生性愈伤组织和随后的芽诱导。在补充了4 mg L ^-1 2iP，0.5 mg L ^-1 BA和0.1 mg L ^-1 NAA的MS培养基上，每个愈伤组织最多获得6.84±0.05个芽。当在补充有2 mg L ^-1吲哚-3-丁酸（IBA）的半强度MS培养基上培养时，子弹产生根。体外繁殖的小植株在土壤上硬化并适应田间条件，存活率达85％。适应植物的叶绿素含量与母体植物相当，而再生植物的气孔微观形态没有异常。用2,2-二苯基-1-吡啶并肼基（DPPH），血浆铁还原能力（FRAP）和磷钼测试测定了叶片甲醇提取物的自由基清除和抗氧化活性。在所有实验中，再生植物均表现出增强的抗氧化潜能，表明微繁殖的植物可用于分离新型生物分子。此外，通过基于PCR的起始密码子靶向（SCoT）标记和ycf1证实了驯化植物的遗传同质性。b DNA条形码引物表现出与正常母本植物相同的单态性条带，未观察到变异。