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A method for topical dosing of invertebrates with pesticide for use in feeding experiments
Ecotoxicology ( IF 2.7 ) Pub Date : 2021-01-14 , DOI: 10.1007/s10646-020-02324-y
Paul Story , Kristine French , Kimberly Maute , Andrew C. Warden , Greg Dojchinov , Grant C. Hose

The ability to produce large numbers of pesticide-exposed insects (e.g. crickets) is important for feeding studies into the effects of pesticides on key predatory species. House crickets (Acheta domesticus L. 1758) were submersed in serial dilutions of the pesticides, fenitrothion and fipronil, used for the control of locusts in Australia, and then rapidly frozen for residue analysis. Good correlations were found between increasing concentrations of serial pesticide dilutions and the resultant residual concentrations of the parent compounds in crickets, with R2 values of 0.949 (fenitrothion) and 0.946 (fipronil). R2 values for the much less abundant fipronil metabolites were lower 0.858 (sulfone), 0.368 (desulfinyl) and 0.785 (sulfide). This method enables insecticide exposure mimicking the field conditions to be assessed, and can be done immediately prior to an experiment. This ensures locusts remain alive when introduced to the feeding chambers, and enables multiple prey items to be dosed with a known pesticide burden.



中文翻译:

一种用于饲料喂养的无脊椎动物与农药的局部给药方法

产生大量接触农药的昆虫(例如)的能力对于研究农药对主要掠食性物种的影响至关重要。将家(Acheta domesticu s L. 1758)浸入农药,杀虫硫磷和氟虫腈的系列稀释液中,用于澳大利亚的蝗虫防治,然后迅速冷冻以进行残留分析。在连续农药稀释液的浓度增加与中母体化合物的残留浓度之间存在良好的相关性,R 2值为0.949(杀nitro硫酮)和0.946(氟虫腈)。R 2丰富度较低的氟虫腈代谢产物的值较低,为0.858(砜),0.368(去亚磺酰基)和0.785(硫化物)。该方法可以评估模拟田间条件的杀虫剂暴露,并且可以在实验前立即进行。这样可以确保蝗虫在被引入饲养室时仍能存活,并且可以给多个猎物分配已知的农药负担。

更新日期:2021-01-14
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