Ecotoxicology ( IF 2.7 ) Pub Date : 2021-01-14 , DOI: 10.1007/s10646-020-02336-8 Hongxing Yang 1, 2 , Yanlin Zhang 1 , Shaochuang Chuang 1 , Weimiao Cao 1 , Zhepu Ruan 1 , Xihui Xu 1 , Jiandong Jiang 1
Bioaugmentation, a strategy based on microbiome engineering, has been proposed for bioremediation of pollutant-contaminated environments. However, the complex microbiome engineering processes for soil bioaugmentation, involving interactions among the exogenous inoculum, soil environment, and indigenous microbial microbiome, remain largely unknown. Acetamiprid is a widely used neonicotinoid insecticide which has caused environmental contaminations. Here, we used an acetamiprid-degrading strain, Pigmentiphaga sp. D-2, as inoculum to investigate the effects of bioaugmentation on the soil microbial community and the process of microbiome reassembly. The bioaugmentation treatment removed 94.8 and 92.5% of acetamiprid within 40 days from soils contaminated with 50 and 200 mg/kg acetamiprid, respectively. A decrease in bacterial richness and diversity was detected in bioaugmentation treatments, which later recovered with the removal of acetamiprid from soil. Moreover, the bioaugmentation treatment significantly influenced the bacterial community structure, whereas application of acetamiprid alone had little influence on the soil microbial community. Furthermore, the bioaugmentation treatment improved the growth of bacteria associated with acetamiprid degradation, and the inoculated and recruited taxa significantly influenced the keystone taxa of the indigenous microbiome, resulting in reassembly of the bacterial community yielding higher acetamiprid-degrading efficiency than that of the indigenous and acetamiprid-treated communities. Our results provide valuable insights into the mechanisms of microbiome engineering for bioaugmentation of acetamiprid-contaminated soils.
中文翻译:
用 Pigmentiphaga sp. 生物强化啶虫脒污染的土壤。菌株 D-2 及其对土壤微生物群落的影响
生物强化是一种基于微生物组工程的策略,已被提议用于污染物污染环境的生物修复。然而,土壤生物强化的复杂微生物组工程过程,涉及外源接种物、土壤环境和本地微生物微生物组之间的相互作用,在很大程度上仍然未知。啶虫脒是一种广泛使用的新烟碱类杀虫剂,已引起环境污染。在这里,我们使用了啶虫脒降解菌株Pigmentiphagasp. D-2,作为接种物,研究生物强化对土壤微生物群落和微生物组重组过程的影响。生物强化处理在 40 天内分别从被 50 和 200 毫克/千克啶虫脒污染的土壤中去除了 94.8% 和 92.5% 的啶虫脒。在生物强化处理中检测到细菌丰富度和多样性下降,后来随着从土壤中去除啶虫脒而恢复。此外,生物强化处理显着影响了细菌群落结构,而单独施用啶虫脒对土壤微生物群落几乎没有影响。此外,生物强化处理改善了与啶虫脒降解相关的细菌的生长,接种和招募的分类群显着影响了土著微生物群落的关键分类群,导致细菌群落的重组产生比土著和啶虫脒处理的群落更高的啶虫脒降解效率。我们的研究结果为微生物组工程对啶虫脒污染土壤的生物强化机制提供了宝贵的见解。