As a result of the increasing demand for edible oils, which are an important part of human nutrition, in recent years, serious environmental problems may arise both during the production and after consumption of these oils. One of these problems is the removal of oily wastewater formed by mixing these oils with water. In this study, lipase enzyme produced by Acinetobacter haemolyticus was immobilized onto eggshell membrane (ESM) using adsorption and cross-linking methods, and optimization and characterization studies of lipase immobilization were performed. ESM and lipase immobilized ESM were characterized by Fourier Transform Infrared (FTIR) Spectroscopy and Scanning Electron Microscopy (SEM). At 50 °C, 60 °C and 70 °C, the immobilized enzyme retained 50% of its activity for 150, 120 and 80 min, respectively. In pH stability experiments, the immobilized enzyme showed much higher activity, especially in the acidic region, compared to the free enzyme. The immobilized enzyme managed to preserve approximately 50% of its activity after 19 reuses. As a result, it can be said that a new, economical, reusable and alternative bioremediation method has been developed by using lipase immobilized ESM in the treatment of wastewater containing oils with high oleic acid composition.

中文翻译：

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一种新的生物修复方法，用于去除油酸成分较高的含油废水：溶血不动杆菌脂肪酶固定在蛋壳膜上，具有更高的稳定性

由于对食用油的需求不断增加，食用油是人类营养的重要组成部分，近年来，在这些油的生产过程中和食用后，都可能出现严重的环境问题。这些问题之一是去除通过将这些油与水混合而形成的含油废水。在这项研究中，溶血不动杆菌产生的脂肪酶用吸附和交联方法将其固定在蛋壳膜（ESM）上，并进行了脂肪酶固定化的优化和表征研究。通过傅立叶变换红外（FTIR）光谱和扫描电子显微镜（SEM）对ESM和固定化脂肪酶的ESM进行了表征。在50°C，60°C和70°C下，固定化酶分别在150、120和80分钟内保留其活性的50％。在pH稳定性实验中，与游离酶相比，固定化酶表现出更高的活性，尤其是在酸性区域。重复使用19次后，固定化酶设法保留了大约50％的活性。结果，可以说是一种新颖，经济，