当前位置: X-MOL 学术Appl. Phys. Express › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Layer formation of 2,5-dihydroxybenzoic acid crystals via mist deposition for mass spectrometry imaging of biological samples
Applied Physics Express ( IF 2.3 ) Pub Date : 2021-01-13 , DOI: 10.35848/1882-0786/abd6a1
Yuji Nakabayashi 1 , Heisuke Sakai 2 , Akio Miyazato 1 , Chiko Shijimaya 1 , Ryoichi Suzuki 3 , Satoru Yamada 4
Affiliation  

Dihydroxybenzoic acid (DHB) crystal layers were formed via mist deposition. Crystal layers exhibiting whiskers measuring a few hundred micrometers were formed at a nozzle temperature of 200 C. DHB crystal layers exhibited strong absorbance in the ultra-violet wavelength regions, and Raman spectroscopy confirmed their vibrational property. The lipid component was analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry imaging using a DHB crystal layer as the mass ionization assist agent. Cholesterols, diacylglycerides, and triglycerides were detected as sodium adducts. By preventing heterogeneous co-crystallization with analytes, mass images were clearer than when using the conventional aerosol spray method.



中文翻译:

通过雾化沉积形成2,5-二羟基苯甲酸晶体的层用于生物样品的质谱成像

二羟基苯甲酸(DHB)晶体层是通过薄雾沉积形成的。在200℃的喷嘴温度下形成具有几百微米的晶须的晶体层。DHB晶体层在紫外波长区域中显示出强吸收性,并且拉曼光谱证实了它们的振动特性。使用DHB晶体层作为质量电离助剂,通过基质辅助激光解吸/电离飞行时间质谱成像分析脂质成分。胆固醇,甘油二酸酯和甘油三酸酯被检测为钠加合物。通过防止与分析物的异质共结晶,质量图像比使用常规气溶胶喷雾方法时更清晰。

更新日期:2021-01-13
down
wechat
bug