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Dynamics of delivering aptamer targeted nano-drugs into cells
Journal of Materials Chemistry B ( IF 7 ) Pub Date : 2021-1-4 , DOI: 10.1039/d0tb02527e
Yulin Liu 1, 2, 3, 4, 5 , Yu Yang 1, 2, 3, 4, 5 , Qingrong Zhang 1, 2, 3, 4, 5 , Denghua Lu 1, 2, 3, 4, 5 , Siying Li 1, 2, 3, 4, 5 , Junfeng Li 1, 2, 3, 4, 5 , Guocheng Yang 1, 2, 3, 4, 5 , Yuping Shan 1, 2, 3, 4, 5
Affiliation  

A targeted nano-drug delivery system has provided great potential and benefits to the diagnosis and therapy of cancers. Cell entry is a critical step for taking effect of the targeted nano-drug. In this report, the dynamics of delivering a single aptamer targeted polyamindoamine–camptothecin–AS1411 (PAMAM–CPT–AS1411) nano-drug into cells was investigated using a force tracing technique based on atomic force microscopy. The results show that the specific interaction of AS1411 and nucleolin, which is overexpressed on cancer cells, enhances the efficiency of the PAMAM–CPT–AS1411 cell entry. Moreover, the specific interaction induced receptor-mediated endocytosis prolongs the duration and decreases the speed of a single PAMAM–CPT–AS1411 cell entry, which is helpful to understand the targeted nano-drugs prolonging the therapeutic drug level. However, the required force for PAMAM–CPT–AS1411 cell entry is not changed. This report will provide a novel and potential method for achieving the precise dynamics of targeted nano-drug delivery.

中文翻译:

将适体靶向的纳米药物递送到细胞中的动力学

靶向纳米药物输送系统为癌症的诊断和治疗提供了巨大的潜力和益处。细胞进入是实现靶向纳米药物作用的关键步骤。在本报告中,使用了基于原子力显微镜的力追踪技术,研究了将单个适体靶向的聚氨基胺-喜树碱-AS1411(PAMAM-CPT-AS1411)纳米药物递送到细胞中的动力学。结果表明,在癌细胞中过表达的AS1411和核仁素的特异性相互作用提高了PAMAM–CPT–AS1411细胞进入的效率。此外,特异性相互作用诱导的受体介导的内吞作用会延长单个PAMAM–CPT–AS1411的持续时间并降低其速度细胞进入,这有助于了解延长治疗药物水平的靶向纳米药物。但是,PAMAM–CPT–AS1411单元进入所需的力没有改变。该报告将为实现靶向纳米药物递送的精确动力学提供一种新颖且潜在的方法。
更新日期:2021-01-13
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