The Spike protein (S protein) is a critical component in the infection of the new coronavirus (SARS-CoV-2). The objective of this work was to evaluate whether peptides from S protein could cause negative impact in the aquatic animals. The aquatic toxicity of SARS-CoV-2 spike protein peptides derivatives has been evaluated in tadpoles (n = 50 tadpoles / 5 replicates of 10 animals) from species Physalaemus cuvieri (Leptodactylidae). After synthesis, purification, and characterization of peptides (PSDP2001, PSDP2002, PSDP2003) an aquatic contamination has been simulatedwith these peptides during 24 hours of exposure in two concentrations (100 and 500 ng/mL). The control group (C) was composed of tadpoles kept in polyethylene containers containing de-chlorinated water. Oxidative stress, antioxidant biomarkers and neurotoxicity activity were assessed. In both concentrations, PSPD2002 and PSPD2003 increased catalase and superoxide dismutase antioxidants enzymes activities, as well as oxidative stress (nitrite levels, hydrogen peroxide and reactive oxygen species). All three peptides also increased acetylcholinesterase activity in the highest concentration. These peptides showed molecular interactions in silico with acetylcholinesterase and antioxidant enzymes. Aquatic particle contamination of SARS-CoV-2 has neurotoxics effects in P. cuvieri tadpoles. These findings indicate that the COVID-19 can constitute environmental impact or biological damage potential.

中文翻译：

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通过暴露环境深入了解刺突碎片SARS-CoV-2的神经毒性和毒性：对水生健康的威胁？

穗蛋白（S蛋白）是新型冠状病毒（SARS-CoV-2）感染的关键组成部分。这项工作的目的是评估S蛋白的肽是否会对水生动物造成负面影响。已在来自Phys（Physalaemus cuvieri）（species科）物种的t中评估了SARS-CoV-2突突蛋白肽衍生物的水生毒性（n = 50 // 10只动物的5个重复）。在合成，纯化和表征肽（PSDP2001，PSDP2002，PSDP2003）后，已经在两种浓度（100和500 ng / mL）的暴露过程中用这些肽模拟了水生污染物。对照组（C）由t组成，这些in保存在装有去氯水的聚乙烯容器中。评估氧化应激，抗氧化剂生物标志物和神经毒性活性。在这两种浓度下，PSPD2002和PSPD2003均可增加过氧化氢酶和超氧化物歧化酶抗氧化剂的酶活性，以及​​氧化应激（亚硝酸盐水平，过氧化氢和活性氧物质）。在最高浓度下，所有这三种肽还增加了乙酰胆碱酯酶的活性。这些肽在计算机上显示出与乙酰胆碱酯酶和抗氧化酶的分子相互作用。SARS-CoV-2的水生颗粒污染在cu.cuvieri t中具有神经毒性作用。这些发现表明，COVID-19可能构成环境影响或潜在的生物破坏。在最高浓度下，所有这三种肽还增加了乙酰胆碱酯酶的活性。这些肽在计算机上显示出与乙酰胆碱酯酶和抗氧化酶的分子相互作用。SARS-CoV-2的水生颗粒污染在cu.cuvieri t中具有神经毒性作用。这些发现表明，COVID-19可能构成环境影响或潜在的生物破坏。在最高浓度下，所有这三种肽还增加了乙酰胆碱酯酶的活性。这些肽在计算机上显示出与乙酰胆碱酯酶和抗氧化酶的分子相互作用。SARS-CoV-2的水生颗粒污染在cu.cuvieri t中具有神经毒性作用。这些发现表明，COVID-19可能构成环境影响或潜在的生物破坏。