Abstract

Cell migration and invasion are two essential processes during cancer metastasis. Increasing evidence has shown that the Piezo1 channel is involved in mediating cell migration and invasion in some types of cancers. However, the role of Piezo1 in the breast cancer and its underlying mechanisms have not been clarified yet. Here, we show that Piezo1 is high-expressed in breast cancer cell (BCC) lines, despite its complex expression in clinical patient database. Piezo1 knockdown (Piezo1-KD) promotes unconfined BCC migration, but impedes confined cell migration. Piezo1 may mediate BCC migration through the balances of cell adhesion, cell stiffness, and contractility. Furthermore, Piezo1-KD inhibits BCC invasion by impairing the invadopodium formation and suppressing the expression of metalloproteinases (MMPs) as well. However, the proliferation and cell cycle of BCCs are not significantly affected by Piezo1. Our study highlights a crucial role of Piezo1 in regulating migration and invasion of BCCs, indicating Piezo1 channel might be a new prognostic and therapeutic target in BCCs.

中文翻译：

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Piezo1通过调节细胞力学生物学特性来调节乳腺癌细胞的迁移和侵袭

摘要

细胞迁移和侵袭是癌症转移过程中的两个基本过程。越来越多的证据表明，Piezo1通道参与介导某些类型癌症中的细胞迁移和侵袭。但是，尚未阐明Piezo1在乳腺癌中的作用及其潜在机制。在这里，我们显示Piezo1在乳腺癌细胞（BCC）系中高表达，尽管其在临床患者数据库中表达复杂。Piezo1敲低（Piezo1-KD）促进了无限制的BCC迁移，但阻碍了受限的细胞迁移。Piezo1可能通过细胞粘附，细胞刚度和收缩力的平衡介导BCC迁移。此外，Piezo1-KD通过削弱侵染脚鸦头的形成并抑制金属蛋白酶（MMPs）的表达来抑制BCC侵袭。然而，Piezo1对BCC的增殖和细胞周期没有显着影响。我们的研究突出了Piezo1在调节BCC迁移和侵袭中的关键作用，表明Piezo1通道可能是BCC中新的预后和治疗靶标。