Smooth muscles are a specific muscle subtype that is widely identified in the tissues of internal passageways. This muscle subtype has the capacity for controlled or regulated contraction and relaxation. Airway smooth muscles are a unique type of smooth muscles that constitute the effective, adjustable, and reactive wall that covers most areas of the entire airway from the trachea to lung tissues. Infection with SARS-CoV-2, which caused the world-wide COVID-19 pandemic, involves airway smooth muscles and their surrounding inflammatory environment. Therefore, airway smooth muscles and related inflammatory factors may play an irreplaceable role in the initiation and progression of several severe diseases. Many previous studies have attempted to reveal the potential relationships between interleukins and airway smooth muscle cells only on the omics level, and the continued existence of numerous false-positive optimal genes/transcripts cannot reflect the actual effective biological mechanisms underlying interleukin-based activation effects on airway smooth muscles. Here, on the basis of newly presented machine learning-based computational approaches, we identified specific regulatory factors and a series of rules that contribute to the activation and stimulation of airway smooth muscles by IL-13, IL-17, or the combination of both interleukins on the epigenetic and/or transcriptional levels. The detected discriminative factors (genes) and rules can contribute to the identification of potential regulatory mechanisms linking airway smooth muscle tissues and inflammatory factors and help reveal specific pathological factors for diseases associated with airway smooth muscle inflammation on multiomics levels.

平滑肌是在内部通道组织中广泛发现的一种特定的肌肉亚型。这种肌肉亚型具有控制或调节收缩和松弛的能力。气道平滑肌是一种独特的平滑肌，它构成有效，可调节和反应性的壁，覆盖从气管到肺组织的整个气道的大部分区域。SARS-CoV-2感染引起世界范围的COVID-19大流行，涉及气道平滑肌及其周围的炎症环境。因此，气道平滑肌和相关的炎性因子可能在几种严重疾病的发生和发展中起着不可替代的作用。以前的许多研究试图仅在组学水平上揭示白细胞介素与气道平滑肌细胞之间的潜在关系，并且许多假阳性的最佳基因/转录本的持续存在无法反映出基于白介素的激活作用的实际有效生物学机制。气道平滑肌。在此，基于新近提出的基于机器学习的计算方法，我们确定了特定的调节因素和一系列规则，这些规则和因子通过IL-13，IL-17或两者的结合来激活和刺激气道平滑肌表观遗传和/或转录水平的白介素。