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Polyhydroxyalkanoate Synthesis by Burkholderia glumae into a Sustainable Sugarcane Biorefinery Concept
Frontiers in Bioengineering and Biotechnology ( IF 5.7 ) Pub Date : 2021-01-13 , DOI: 10.3389/fbioe.2020.631284
Carolina Bilia Chimello de Paula , Fabrício Coutinho de Paula-Elias , Marcela Nogueira Rodrigues , Luciana Fontes Coelho , Nayra Morgana Lima de Oliveira , Alex Fernando de Almeida , Jonas Contiero

Polyhydroxyalkanoate (PHA) bioplastic was synthesized by Burkholderia glumae MA13 from carbon sources and industrial byproducts related to sugarcane biorefineries: sucrose, xylose, molasses, vinasse, bagasse hydrolysate, yeast extract, yeast autolysate, and inactivated dry yeast besides different inorganic nitrogen sources. Sugarcane molasses free of pre-treatment was the best carbon source, even compared to pure sucrose, with intracellular polymer accumulation values of 41.1–46.6% cell dry weight. Whereas, xylose and bagasse hydrolysate were poor inducers of microbial growth and polymer synthesis, the addition of 25% (v/v) sugarcane vinasse to the culture media containing molasses was not deleterious and resulted in a statistically similar maximum polymer content of 44.8% and a maximum PHA yield of 0.18 g/g, at 34°C and initial pH of 6.5, which is economic and ecologically interesting to save water required for the industrial processes and especially to offer a fermentative recycling for this final byproduct from bioethanol industry, as an alternative to its inappropriate disposal in water bodies and soil contamination. Ammonium sulfate was better even than tested organic nitrogen sources to trigger the PHA synthesis with polymer content ranging from 29.7 to 44.8%. GC-MS analysis showed a biopolymer constituted mainly of poly(3-hydroxybutyrate) although low fractions of 3-hydroxyvalerate monomer were achieved, which were not higher than 1.5 mol% free of copolymer precursors. B. glumae MA13 has been demonstrated to be adapted to synthesize bioplastics from different sugarcane feedstocks and corroborates to support a biorefinery concept with value-added green chemicals for the sugarcane productive chain with additional ecologic benefits into a sustainable model.

中文翻译:

Burkholderia glumae 将聚羟基链烷酸酯合成为可持续的甘蔗生物精炼概念

聚羟基链烷酸酯 (PHA) 生物塑料由 Burkholderia glumae MA13 从与甘蔗生物精炼相关的碳源和工业副产品合成:蔗糖、木糖、糖蜜、酒糟、甘蔗渣水解物、酵母提取物、酵母自溶物和灭活的干酵母,以及不同的无机氮源。未经预处理的甘蔗糖蜜是最好的碳源,即使与纯蔗糖相比,细胞内聚合物积累值为 41.1-46.6% 细胞干重。然而,木糖和甘蔗渣水解物是微生物生长和聚合物合成的不良诱导剂,将 25% (v/v) 甘蔗酒糟添加到含有糖蜜的培养基中无害,并导致 44.8% 和 44.8% 的最大聚合物含量在统计上相似。在 34°C 和初始 pH 值为 6.5 时,最大 PHA 产量为 0.18 g/g,节约工业过程所需的水,特别是为生物乙醇工业的最终副产品提供发酵回收,作为其在水体和土壤污染中的不当处置的替代方案,这在经济和生态上都是有意义的。硫酸铵甚至比测试的有机氮源更好地触发 PHA 合成,聚合物含量范围为 29.7 至 44.8%。GC-MS 分析显示生物聚合物主要由聚(3-羟基丁酸酯)构成,尽管实现了低比例的 3-羟基戊酸酯单体,不高于 1.5 mol%,不含共聚物前体。B.
更新日期:2021-01-13
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