Acidithiobacillus ferridurans JAGS is a newly isolated acidophile from an acid mine drainage (AMD). The genome of isolate JAGS was sequenced and compared with eight other published genomes of Acidithiobacillus. The pairwise mutation distance (Mash) and average nucleotide identity (ANI) revealed that isolate JAGS had a close evolutionary relationship with A. ferridurans JCM18981, but whole-genome alignment showed that it had higher similarity in genomic structure with A. ferrooxidans species. Pan-genome analysis revealed that nine genomes were comprised of 4601 protein coding sequences, of which 43% were core genes (1982) and 23% were unique genes (1064). A. ferridurans species had more unique genes (205–246) than A. ferrooxidans species (21–234). Functional gene categorizations showed that A. ferridurans strains had a higher portion of genes involved in energy production and conversion while A. ferrooxidans had more for inorganic ion transport and metabolism. A high abundance of kdp, mer and ars genes, as well as mobile genetic elements, was found in isolate JAGS, which might contribute to its resistance to harsh environments. These findings expand our understanding of the evolutionary adaptation of Acidithiobacillus and indicate that A. ferridurans JAGS is a promising candidate for biomining and AMD biotreatment applications.

中文翻译：

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一种新分离的铁酸铁硫杆菌JAGS菌株的基因组分析揭示了其对酸性矿山排水的适应性

Ferridurans嗜酸硫杆菌JAGS是从酸性矿山排水系统（AMD）中分离出来的新嗜酸菌。分离的JAGS基因组进行了测序，并与其他8个已发表的酸性硫杆菌基因组进行了比较。成对突变距离（醪）和平均核苷酸同一性（ANI）表明分离株JAGS曾与密切的进化关系A. ferridurans JCM18981，但全基因组比对显示，它在基因组结构相似性较高与A.杆菌物种。泛基因组分析显示9个基因组由4601个蛋白质编码序列组成，其中43％是核心基因（1982年）和23％是独特基因（1064年）。A. ferridurans物种具有比（205-246）更多的独特基因A. ferrooxidans种（21–234）。功能基因分类已表明，A. ferridurans菌株具有参与能量生产和转化率，同时基因的较高部分A.杆菌有更多的无机离子转运和代谢。在分离的JAGS中发现了大量的kdp，mer和ars基因，以及可移动的遗传元件，这可能有助于其抵抗恶劣环境。这些发现扩大了我们对酸性硫杆菌的进化适应性的理解，并表明铁曲霉JAGS是生物采矿和AMD生物处理应用的有希望的候选者。