The occurrence and diversity of Lecanicillium and Sarocladium in maize seeds and their role in this cereal are poorly understood. Therefore, the present study aimed to investigate Sarocladium and Lecanicillium communities found in endosphere of maize seeds collected from fields in Poland and their potential to form selected bioactive substances. The sequencing of the internally transcribed spacer regions 1 (ITS 1) and 2 (ITS2) and the large-subunit (LSU, 28S) of the rRNA gene cluster resulted in the identification of 17 Sarocladium zeae strains, three Sarocladium strictum and five Lecanicillium lecanii isolates. The assay on solid substrate showed that S. zeae and S. strictum can synthesize bassianolide, vertilecanin A, vertilecanin A methyl ester, 2-decenedioic acid and 10-hydroxy-8-decenoic acid. This is also the first study revealing the ability of these two species to produce beauvericin and enniatin B1, respectively. Moreover, for the first time in the present investigation, pyrrocidine A and/or B have been annotated as metabolites of S. strictum and L. lecanii. The production of toxic, insecticidal and antibacterial compounds in cultures of S. strictum, S. zeae and L. lecanii suggests the requirement to revise the approach to study the biological role of fungi inhabiting maize seeds.

中文翻译：

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Sarocladium和Lecanicillium与玉米种子相关联并具有形成某些次生代谢产物的潜力

人们对玉米种子中Lecanicillium和Sarocladium的发生和多样性及其在这种谷物中的作用了解得很少。因此，本研究旨在调查在波兰田间收集的玉米种子的内层中发现的Sarocladium和Lecanicillium群落及其形成选定生物活性物质的潜力。对内部转录的间隔区1（ITS 1）和2（ITS2）以及rRNA基因簇的大亚基（LSU，28S）进行测序，结果鉴定出17株Sarocladium zeae菌株， 3株Sarocladiumstrictum和5株Lecanicillium lecanii隔离株。固体基板上的检测表明S.玉蜀黍赤霉和S. strictum可以合成白僵菌，vertilecanin A，vertilecanin甲甲基酯，2-癸烯二酸和10-羟基-8-癸烯酸。这也是第一个揭示这两个物种分别产生beauvericin和enniatin B1的能力的研究。此外，在本调查中，pyrrocidine A和/或B中的第一次已经被注释为代谢物S. strictum和L.枝菌三级。在S.strictum，S。zeae和L. lecanii的培养物中产生有毒，杀虫和抗菌化合物建议修改研究居住在玉米种子中的真菌的生物学作用的方法的要求。