The implementation of Boolean logic circuits in cells have become a very active field within synthetic biology. Although these are mostly focussed on the genetic components alone, the context in which the circuit performs is crucial for its outcome. We characterise 20 genetic NOT logic gates in up to 7 bacterial-based contexts each, to generate 135 different functions. The contexts we focus on are combinations of four plasmid backbones and three hosts, two Escherichia coli and one Pseudomonas putida strains. Each gate shows seven different dynamic behaviours, depending on the context. That is, gates can be fine-tuned by changing only contextual parameters, thus improving the compatibility between gates. Finally, we analyse portability by measuring, scoring, and comparing gate performance across contexts. Rather than being a limitation, we argue that the effect of the genetic background on synthetic constructs expands functionality, and advocate for considering context as a fundamental design parameter.

在细胞中实现布尔逻辑电路已成为合成生物学中一个非常活跃的领域。尽管这些主要集中在遗传成分上，但电路执行的环境对其结果至关重要。我们在多达 7 个基于细菌的上下文中描述了 20 个遗传非逻辑门，以生成 135 种不同的功能。我们关注的背景是四个质粒骨架和三个宿主、两个大肠杆菌和一个恶臭假单胞菌的组合菌株。每个门显示七种不同的动态行为，具体取决于上下文。也就是说，可以通过仅更改上下文参数来微调门，从而提高门之间的兼容性。最后，我们通过测量、评分和比较跨上下文的门性能来分析可移植性。我们认为遗传背景对合成结构的影响扩展了功能，而不是限制，并提倡将上下文视为基本设计参数。