ABSTRACT

The disinfection efficiency of ozonation by ozonated water on human norovirus genogroup-II (HNoV-II) and MS2 as a surrogate was investigated using plaque assay and/or quantitative PCR (qPCR). The qPCR combining propidium monoazide (PMA) pretreatment was used to evaluate the damage to the viral capsids, which showed that the PMA pretreatment could be applied to HNoV-II but not to MS2 because PMA inhibited qPCR even in undamaged MS2. The ozonation reduced the viability of MS2 by more than 99.9999% (= 6 log; CT = 0.036 mgXmin/L) and the qPCR detection of HNoV-II by approximately 90–95%. However, it is assumed that significant damage to the HNoV-II capsid did not occur in all inactivated viruses.

摘要

使用空斑分析和/或定量PCR（qPCR）研究了臭氧水对人诺如病毒基因组II（HNoV-II）和MS2的替代臭氧消毒效率。qPCR结合单叠氮化丙锭（PMA）预处理用于评估病毒衣壳的损伤，这表明PMA预处理可以应用于HNoV-II，但不能应用于MS2，因为即使在未损坏的MS2中，PMA也会抑制qPCR。臭氧化作用使MS2的活力降低了99.9999％（= 6 log； CT = 0.036 mgXmin / L），qPCR检测到的HNoV-II降低了约90-95％。但是，假定在所有灭活的病毒中均未发生对HNOV-II衣壳的重大破坏。