Abstract

Current study was formulated to analyze the chemical composition, pharmacological activities, and toxicology study of Chromolaena odorata aerial part essential oil (COEO) grown in North East India. Gas Chromatography/Mass Spectroscopy was used for chemical composition analysis. The antioxidative potential was tested using DPPH and reducing power assays. Protein denaturation and protease inhibitor assays for anti-inflammatory activity and Allium cepa assay were used to test the genotoxicity of the essential oil. Standard disc diffusion and minimum inhibitory assays (MIC) were employed to test the antimicrobial efficacy of COEO and Acetylcholinesterase (AChE) inhibitory assay was used for anticholinesterase activity. GC/MS analysis revealed Geijerene (26.34 %), α-Copaene (17.87 %), Caryophyllene (11.14 %), 3-Carene (10.63 %), Cadinene (7.98 %), Cyclohexane, 1-Methylene-4-(1-methylethenyl)-(3.37 %), Elemol (2.89 %), τ-Muurolol (2.77 %), as major components. Standard DPPH, protein denaturation, and protease inhibitor assays revealed (IC₅₀= 15.88 μg/mL, 19.35 μg/mL, and 27.28 μg/mL respectively) strong antioxidant, anti-inflammatory potential of COEO. Allium cepa assay showed negative genotoxicity with (mitotic index) MI=16.38 %, which is close to distilled water MI=19.20 %. While disc diffusion assay (zone of inhibition for B.cereus 16.56 ± 0.002 mm; S. aureus 14.20 ± 0.057 mm; S. cerevisiae 10.67 ± 0.015 mm; A. fumigatus 10.15 ± 0.05 mm) and MIC (B. subtilis, S. aureus 2.50 μL/mL), showed the antimicrobial potential of COEO. Strong AChE inhibitory activity was observed with an IC₅₀ value of 61.98 μg/mL. From all the results obtained it is clear that COEO could be used for future research, development, and in preparation of cost-effective pharmacologically important herbal drugs after proper validations.

摘要

制定了当前的研究报告，以分析印度东北部种植的香樟（Chromolaena odorata）地上部分精油（COEO）的化学成分，药理活性和毒理学研究。气相色谱/质谱用于化学成分分析。使用DPPH和还原能力测定法测试抗氧化能力。蛋白变性和蛋白酶抑制剂测定的抗炎活性和洋葱测定法被用于测试精油的遗传毒性。采用标准椎间盘扩散和最小抑制试验（MIC）来测试COEO的抗菌功效，而乙酰胆碱酯酶（AChE）抑制试验则用于抗胆碱酯酶的活性。GC / MS分析显示，Geijerene（26.34％），α-Copaene（17.87％），Caryophyllene（11.14％），3-Carene（10.63％），Cadinene（7.98％），环己烷，1-Methyl-4-（1-主要成分为：甲基乙烯基）-（3.37％），Elemol（2.89％），τ-Muurolol（2.77％）。标准DPPH，蛋白质变性和蛋白酶抑制剂测定显示（IC ₅₀分别为15.88μg/ mL，19.35μg/ mL和27.28μg/ mL）具有很强的抗氧化剂和COEO的抗炎潜力。洋葱检测显示出负的遗传毒性，（有丝分裂指数）MI = 16.38％，接近蒸馏水MI = 19.20％。同时进行椎间盘扩散测定（蜡状芽孢杆菌的抑制区16.56±0.002 mm;金黄色葡萄球菌14.20±0.057 mm;酿酒酵母10.67±0.015 mm;烟曲霉10.15±0.05 mm）和MIC（枯草芽孢杆菌，S。金黄色葡萄球菌2.50μL/ mL）具有COEO的抗菌潜力。观察到强的AChE抑制活性，IC ₅₀值为61.98μg/ mL。从获得的所有结果中可以明显看出，经过适当的验证，COEO可用于未来的研究，开发和制备具有成本效益的具有重要药理意义的草药。