Abstract

The aim of this study was to analyze the essential oils of Myrtus communis L.from the south of Turkey by the GC/MS method and to demonstrate its proapoptotic effects on A549 cells. A549 cells were used as cell lines in the study. Cells were treated for 24 hours with a different concentration of the essential oil (from 200 to 31.25 μg/ml). Non-toxic concentrations of essential oils of MC were determined by the MTT method. Apoptosis studies were performed within the determined non-toxic concentrations of essential oils. Expression levels of the caspase 3, caspase 8, caspase 9, Bcl-2, and p21 genes were performed by qRT-PCR method. Eucalyptol (1,8-cineole; 31.2 %) was found at the highest rate in the essential oils content, while alphapinene followed it with a rate of 29.6 %. Our results showed that essential oil concentrations of 625 μg/ml and higher than this concentration significantly suppressed cell viability at the end of the 24-hour incubation compared to the control group. The expression levels of caspase 3 and caspase 9 genes, which are the mediators of the intrinsic pathway, increased statistically significantly at the essential oil concentration of 312.5 μg/ml (respectively, p=0.0129 and p=0.0180). The expression level of the p21 gene was significantly increased at a concentration of 156.25 μg/ml essential oils (p=0.0002). The essential oils of MC from the South of Turkey have been found to induce apoptosis intrinsically. These findings could be a guide in understanding the molecular mechanism underlying the anti-cancer properties of MC’s essential oils on A549 cells. We think that it has the potential to be a new natural therapeutic agent for cancer treatment.

摘要

这项研究的目的是分析桃金娘的精油通过GC / MS方法从土耳其南部提取L. L.并证明其对A549细胞的促凋亡作用。在研究中将A549细胞用作细胞系。用不同浓度的精油（200至31.25μg/ ml）处理细胞24小时。通过MTT法测定MC的无毒浓度。在确定的无毒精油浓度内进行凋亡研究。通过qRT-PCR方法进行胱天蛋白酶3，胱天蛋白酶8，胱天蛋白酶9，Bcl-2和p21基因的表达水平。桉树油（1,8-桉树脑； 31.2％）的香精油含量最高，而αpinepinene以29.6％的比例紧随其后。我们的结果表明，与对照组相比，精油浓度为625μg/ ml并高于该浓度会在24小时孵育结束时显着抑制细胞活力。在固有油浓度为312.5μg/ ml的情况下，内在途径的介质caspase 3和caspase 9基因的表达水平显着增加（分别为p = 0.0129和p = 0.0180）。在精油浓度为156.25μg/ ml的情况下，p21基因的表达水平显着提高（p = 0.0002）。已发现来自土耳其南部的MC精油可内在诱导细胞凋亡。这些发现可能为理解MC精油对A549细胞抗癌特性的分子机制提供指导。