Abstract

Variations in the antidermatophytic activity of Cymbopogon martinii was determined in different temperature and storage periods. The essential oil of C. martinii was obtained by hydrodistillation methods and stored in airtight amber bottles in three different temperature conditions. Longevity testing will be performed after each fourth month of storage against selected fungi for up to two years. MIC was determined by semi-solid agar antifungal testing methods. In the case of Candida albicans MIC of oil increased at room temperature during second (0.4 μl/ml) and fifth testing (0.5 μl/ml) while at 30oC temperature it remains constant up to fifth testing and again increased at sixth testing (0.4 μl/ml). Similar results were also observed against Microsporum fulvum where MIC of oil at room temperature and 30oC temperature increased at fifth testing (0.3 μl/ml). In the case of M. canis MIC was found to be increased at fifth (0.4 μl/ml) and sixth testing (0.5 μl/ml) at 30oC temperature conditions. Trichophyton rubrum was found to be the most resistant fungus. MIC of C. martinii essential oil stored at room temperature was found to be increased against T. mentagrophytes at fifth testing only. Comparative analysis of antifungal activities exhibited very slight increases in MIC during storage. Therefore C. martinii was found to be stable oil and can be used for a long period of storage. The present study concluded that the best condition to preserve the initial composition of C. martinii essential oil is to keep it at low temperature in a fridge or a freezer.

摘要

在的antidermatophytic活性变化香茅martinii在不同的温度和存储时间确定。精油C. martinii通过水蒸气蒸馏的方法获得并存储在气密琥珀色瓶中在三个不同的温度条件。每隔四个月对选定的真菌进行长达两年的寿命测试。MIC通过半固体琼脂抗真菌测试方法确定。在白色念珠菌的情况下，油的MIC在室温下第二次（0.4μl/ ml）和第五次试验（0.5μl/ ml）升高，而在30°C的温度下直至第五次试验均保持恒定，而在第六次试验（0.4μl / ml）。还观察到类似的结果在第五次测试中，在室温和30oC温度下油的MIC升高时，出现了黄粉菌（0.3μl / ml）。对于犬莫氏杆菌，发现在30oC温度条件下，MIC在第五次（0.4μl/ ml）和第六次试验（0.5μl/ ml）时增加。发现红毛癣菌是最具抗性的真菌。的MIC C. martinii在室温下储存精油发现针对要增加须发癣菌在仅第五测试。在储存过程中，抗真菌活性的比较分析显示MIC的增加非常轻微。因此，马氏梭菌被发现是稳定的油，可以长期保存。本研究得出的结论是，保存马丁尼梭菌精油初始成分的最佳条件是将其在冰箱或冰柜中保持低温。