Abstract:

The essential oil obtained by hydro-distillation of leaves of Ocimum basilicum (basil essential oil) was analyzed for its potential to manage the Alternaria alternata causal agent of Alternaria rot of tomato, in vitro and in vivo. Further, the chemical composition of the extracted basil oil was analyzed by the GC/MS. A total of 20 chemical compounds were identified in the hydro-distilled basil essential oil. The primary constituents of the basil essential oil were methyl chavicol (38.2 %) and linalool (28.7 %). In vitro, the basil essential oil successfully constrained the growth and conidia germination of A. alternata. The highest concentration of basil essential oil (10 %) provided maximum growth reduction (88.0 %) of the fungus. Likewise, the highest concentration of basil essential oil (10 %) caused a 90 % reduction in conidia germination. In vivo, the tomatoes treated with 4 % and 6 % basil essential oil had 33.3 % and 55.5 % respectively, reduction in the infected wounds as compared to control (untreated inoculated). Also, there were 52.9 % and 62.3 % less A. alternata infected fruits when treated with 4 % and 6 % basil essential oil respectively as compared to control (untreated inoculated). This proves that the basil essential oil was able to manage the Alternaria rot of tomato successfully. Therefore, the basil essential oil can substitute the synthetic fungicides to manage the Alternaria rot of tomato.

摘要：

通过叶片的加氢蒸馏获得的精油罗勒（罗勒精油）分析其潜在管理链格孢链格孢的致病剂腐番茄的，在体外和体内。此外，通过GC / MS分析提取的罗勒油的化学组成。在氢化蒸馏的罗勒精油中总共鉴定出20种化合物。罗勒精油的主要成分是甲基查维索尔（38.2％）和芳樟醇（28.7％）。在体外，罗勒精油成功地限制了交链孢霉的生长和分生孢子。罗勒精油的最高浓度（10％）使真菌的最大生长减慢（88.0％）。同样，最高浓度的罗勒精油（10％）导致分生孢子萌发减少了90％。在体内，用4％和6％的罗勒精油处理的西红柿分别具有33.3％和55.5％的感染伤口，与对照相比（未处理的接种物）减少了。另外，交替链球菌也减少了52.9％和62.3％与对照（未经处理的接种物）相比，分别用4％和6％的罗勒精油处理感染的水果。这证明了罗勒精油能够成功地控制番茄的烂链霉病。因此，罗勒精油可以替代合成杀真菌剂来控制番茄的烂链霉病。