Center for Complex Biological Systems, University of California, Irvine, Irvine, CA 92697.
Department of Developmental and Cell Biology.
Department of Biomedical Engineering.
Department of Mathematics, and.
Cellular cargos, including lipid droplets and mitochondria, are transported along microtubules using molecular motors such as kinesins. Many experimental and computational studies focused on cargos with rigidly attached motors, in contrast to many biological cargos that have lipid surfaces that may allow surface mobility of motors. We extend a mechanochemical 3D computational model by adding coupled-viscosity effects to compare different motor arrangements and mobilities. We show that organizational changes can optimize for different objectives: Cargos with clustered motors are transported efficiently, but are slow to bind to microtubules, whereas those with motors dispersed rigidly on their surface bind microtubules quickly, but are transported inefficiently. Finally, cargos with freely-diffusing motors have both fast binding and efficient transport, although less efficient than clustered motors. These results suggest that experimentally observed changes in motor organization may be a control point for transport.
Movie S1: Animation from example simulation of cargo with free independent motors. Frames in Fig 2B taken from this simulation.Download Original Video (14.8 MB)https://ascb-prod-streaming.literatumonline.com/journals/content/mboc/0/mboc.ahead-of-print/mbc.e20-10-0658/20210111/media/mc-e20-10-0658-s02.,1920,1200,960,900,768,652,642,.mp4.m3u8?b92b4ad1b4f274c70877518b17abb28b8c6166875a97f369b6d543b680497f5bca01d1632f390ebb6e08bd2a2725a70a05738b03ddc7251bffb1206bcd384e738aacf0abe9b4502e102dfa92763a3cb1ec25a8389f9bc82563cf2e49eb34f3003e2ea6643d05d4c14ec19cee0d4dd93d8f7fd177ac61b8ae00914fc8fd526d24cfd2160bb87518e5397668cce2e26acd3f16fd31c33c1da35fd73058e7cb081e3da0b304bd6e9c649ca3d5b9b2f5cf5c52e83ed8e5d429Movie S2: Animation of cargo binding for cargo with rigid clustered motors. Cargo eventually binds at t = 36 s, full trajectory not shown.Download Original Video (29.7 MB)https://ascb-prod-streaming.literatumonline.com/journals/content/mboc/0/mboc.ahead-of-print/mbc.e20-10-0658/20210111/media/mc-e20-10-0658-s03.,1920,1200,960,900,768,652,642,.mp4.m3u8?b92b4ad1b4f274c70877518b17abb28b8c6166875a97f369b6d543b680497f5bca01d1632f390ebb6e08bd2a2725a70a05738b03ddc7251bffb1206bcd384e738aacf0abe9b4502e102dfa92763a3cb1ec25a8389f9bc82563cf2e49eb34f3003e2ea6643d05d4c14ec19cee0d4dd93d8f7fd177ac61b8ae01914fc8fd526d24d99a94ae6cf3365b25da0f5e2b84a30dae9ac7161d4fc5df8bda0f2d9e4dcc0902a2b3cdc124244469c81fef7884984e486657e2b90b4cMovie S3: Animation of cargo binding for cargo with rigid dispersed motors.Download Original Video (13.9 MB)https://ascb-prod-streaming.literatumonline.com/journals/content/mboc/0/mboc.ahead-of-print/mbc.e20-10-0658/20210111/media/mc-e20-10-0658-s04.,1920,1200,960,900,768,652,642,.mp4.m3u8?b92b4ad1b4f274c70877518b17abb28b8c6166875a97f369b6d543b680497f5bca01d1632f390ebb6e08bd2a2725a70a05738b03ddc7251bffb1206bcd384e738aacf0abe9b4502e102dfa92763a3cb1ec25a8389f9bc82563cf2e49eb34f3003e2ea6643d05d4c14ec19cee0d4dd93d8f7fd177ac61b8ae06914fc8fd526d240e7cd4b6f1ff7d765595c403847fffc91fe9941936fabe33f0ac557d2aa0c8b2e66d11c02e1ae853c9108a12a66c492381d1a758038ee5Movie S4: Animation of cargo binding for cargo with free independent motors.Download Original Video (4.4 MB)https://ascb-prod-streaming.literatumonline.com/journals/content/mboc/0/mboc.ahead-of-print/mbc.e20-10-0658/20210111/media/mc-e20-10-0658-s05.,1920,1200,960,900,768,652,642,.mp4.m3u8?b92b4ad1b4f274c70877518b17abb28b8c6166875a97f369b6d543b680497f5bca01d1632f390ebb6e08bd2a2725a70a05738b03ddc7251bffb1206bcd384e738aacf0abe9b4502e102dfa92763a3cb1ec25a8389f9bc82563cf2e49eb34f3003e2ea6643d05d4c14ec19cee0d4dd93d8f7fd177ac61b8ae07914fc8fd526d249ca28f15fddff79257a7b2a39c46404b9ffbb9d2a225344a44781e0acfcd362a32963894a861d5e1cc1c474738cd24732d4e915f606e03Movie S5: Animation of cargo binding for cargo with free clustered motors.Download Original Video (29.4 MB)https://ascb-prod-streaming.literatumonline.com/journals/content/mboc/0/mboc.ahead-of-print/mbc.e20-10-0658/20210111/media/mc-e20-10-0658-s06.,1920,1200,960,900,768,652,642,.mp4.m3u8?b92b4ad1b4f274c70877518b17abb28b8c6166875a97f369b6d543b680497f5bca01d1632f390ebb6e08bd2a2725a70a05738b03ddc7251bffb1206bcd384e738aacf0abe9b4502e102dfa92763a3cb1ec25a8389f9bc82563cf2e49eb34f3003e2ea6643d05d4c14ec19cee0d4dd93d8f7fd177ac61b8ae04914fc8fd526d247122e24733bb7cf0751569757476a98d5cebe87c3059bc38cf114152931d8b2bf5aff170738bb5b7366cc702963f7af5fc3fca4b7eb1dfMovie S6: Animation of run length for cargo with rigid clustered motors.Download Original Video (4.9 MB)https://ascb-prod-streaming.literatumonline.com/journals/content/mboc/0/mboc.ahead-of-print/mbc.e20-10-0658/20210111/media/mc-e20-10-0658-s07.,1920,1200,960,900,768,652,642,.mp4.m3u8?b92b4ad1b4f274c70877518b17abb28b8c6166875a97f369b6d543b680497f5bca01d1632f390ebb6e08bd2a2725a70a05738b03ddc7251bffb1206bcd384e738aacf0abe9b4502e102dfa92763a3cb1ec25a8389f9bc82563cf2e49eb34f3003e2ea6643d05d4c14ec19cee0d4dd93d8f7fd177ac61b8ae05914fc8fd526d243acc055fb0c6e1a72ec0b45319611990e3cc062a505acae7e2a87f0de6ae6e43b6fed49adc39706baef8a2eaa09d9732af2062d7970367Movie S7: Animation of run length for cargo with rigid dispersed motors.Download Original Video (11.7 MB)https://ascb-prod-streaming.literatumonline.com/journals/content/mboc/0/mboc.ahead-of-print/mbc.e20-10-0658/20210111/media/mc-e20-10-0658-s08.,1920,1200,960,900,768,652,642,.mp4.m3u8?b92b4ad1b4f274c70877518b17abb28b8c6166875a97f369b6d543b680497f5bca01d1632f390ebb6e08bd2a2725a70a05738b03ddc7251bffb1206bcd384e738aacf0abe9b4502e102dfa92763a3cb1ec25a8389f9bc82563cf2e49eb34f3003e2ea6643d05d4c14ec19cee0d4dd93d8f7fd177ac61b8ae0a914fc8fd526d249c3df7fc7d7f96696726a192eca8c34b8f744db7497d772a105193c39ac2ec061fcf6262d0e1b94a76a81ff8ea1ea90139056a8c8dae70Movie S8: Animation of run length for cargo with free independent motors.Download Original Video (9.0 MB)https://ascb-prod-streaming.literatumonline.com/journals/content/mboc/0/mboc.ahead-of-print/mbc.e20-10-0658/20210111/media/mc-e20-10-0658-s09.,1920,1200,960,900,768,652,642,.mp4.m3u8?b92b4ad1b4f274c70877518b17abb28b8c6166875a97f369b6d543b680497f5bca01d1632f390ebb6e08bd2a2725a70a05738b03ddc7251bffb1206bcd384e738aacf0abe9b4502e102dfa92763a3cb1ec25a8389f9bc82563cf2e49eb34f3003e2ea6643d05d4c14ec19cee0d4dd93d8f7fd177ac61b8ae0b914fc8fd526d24268c310e2a8df54d6b6f000bd61cdc575293e131ad692d8db82205ca12f865ea18d388b301dfb8779d3830605f80e03e3110b1cb984a8d
中文翻译:
驱动蛋白马达在货物上的扩散可以增强细胞内运输过程中的结合和运行长度
细胞货物,包括脂滴和线粒体,使用分子马达(如驱动蛋白)沿微管运输。许多实验和计算研究集中在具有刚性连接的电机的货物上,与许多具有脂质表面的生物货物形成对比,这些表面可能允许电机的表面移动。我们通过添加耦合粘度效应来扩展机械化学 3D 计算模型,以比较不同的运动安排和流动性。我们表明,组织变化可以针对不同的目标进行优化:具有集群马达的货物运输有效,但与微管结合的速度较慢,而具有刚性分散在其表面上的马达快速结合微管,但运输效率低下。最后,带有自由扩散电机的货物具有快速绑定和高效运输,但效率不如集群电机。这些结果表明,实验观察到的运动组织变化可能是运输的控制点。