Dysfunctional mitochondrial activity can lead to a variety of different diseases. As such, there exists a need to quantify changes in mitochondria function as it relates to these specific diseased states. Here, we present the use of resonance Raman (RR) spectroscopy as a tool to determine changes in isolated mitochondrial activity. RR spectroscopy, using 532 nm as the excitation source, specifically provides information on the reduction and oxidation (RedOx) state of cytochrome c, which is determined by the activity of protein complexes in the electron transport chain (ETC). In this model, injection of the substrate succinate into the mitochondrial sample is used to drive the ETC, which causes a subsequent change in cytochrome c RedOx state. This change in RedOx state is tracked by RR spectroscopy. This tool gives real‐time information on the rise and fall of the amount of reduced cytochrome c within the mitochondrial sample, providing a method for rapid assessment of mitochondrial metabolism that has broad applications in both basic science and medical research.

中文翻译：

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使用共振拉曼显微光谱连续评估线粒体的代谢活性

功能失调的线粒体活动可导致多种不同的疾病。因此，需要量化与这些特定疾病状态相关的线粒体功能变化。在这里，我们介绍了使用共振拉曼 (RR) 光谱作为确定孤立线粒体活性变化的工具。RR 光谱使用 532 nm 作为激发源，专门提供有关细胞色素 c 的还原和氧化 (RedOx) 状态的信息，该状态由电子传递链 (ETC) 中蛋白质复合物的活性决定。在该模型中，将底物琥珀酸盐注射到线粒体样品中用于驱动 ETC，从而导致细胞色素 c RedOx 状态的后续变化。RedOx 状态的这种变化由 RR 光谱跟踪。