Angiotensin-converting enzyme (ACE) liable for the regulation of blood pressure was purified from human plasma by affinity chromatography. Impact of water and butanol extracts of Matricaria chamomilla L. on purity ACE was examined. ACE was purified using the affinity chromatography method. The enzyme activity was evaluated at 345 nm by a spectrophotometer. Extracts of M. chamomilla plant with butanol and water were prepared. Lisinopril was utilized as a specific inhibitor. ACE was purified 3,659-fold from human plasma and the specific activity was 1,350 EU/mg protein. The molecular weight and purity of ACE were found by SDS-PAGE and two bands of 60 and 70 kDa on the gel were detected. Water and butanol extracts of M. chamomilla demonstrated inhibitor impact on ACE activity. IC₅₀ constants for water and butanol extracts of M. chamomilla were computed to be 1.292 and 0.353 mg/mL, respectively. The type of inhibition for whole inhibitors was identified as noncompetitive. IC₅₀ and K_i constants for lisinopril were calculated to be 0.781 and 0.662 nM, respectively. These results indicate that butanol and water extracts of M. chamomilla may have an ACE inhibitor potential.

中文翻译：

---

洋甘菊丁醇和水提物对人血浆中血管紧张素转化酶抑制作用的研究

通过亲和层析从人血浆中纯化出负责调节血压的血管紧张素转换酶 (ACE)。考察了洋甘菊的水和丁醇提取物对纯度 ACE 的影响。使用亲和色谱法纯化ACE。通过分光光度计在 345 nm 处评估酶活性。用丁醇和水制备洋甘菊植物提取物。赖诺普利被用作特异性抑制剂。ACE 从人血浆中纯化 3,659 倍，比活性为 1,350 EU/mg 蛋白质。用SDS-PAGE测定ACE的分子量和纯度，在凝胶上检测到60和70 kDa的两条条带。M. chamomilla的水和丁醇提取物证明抑制剂对ACE活性的影响。计算出洋甘菊水和丁醇提取物的IC ₅₀常数分别为 1.292 和 0.353 mg/mL。整个抑制剂的抑制类型被确定为非竞争性的。赖诺普利的IC ₅₀和K _i常数经计算分别为 0.781 和 0.662 nM。这些结果表明，洋甘菊的丁醇和水提取物可能具有 ACE 抑制剂的潜力。