Abstract—

The aim of the study is isolation of galactomannan from plant materials and investigation of their physical and chemical properties. Polysaccharides generally consisting of galactomannan were isolated and purified from galactomannans from the seeds of the plants Sophora japonica and Gleditsia triacanthos by cold and hot extraction methods. Galactose and mannose contents of the galactomannans isolated were quantitatively characterized and physical and chemical properties of the polysaccharides were studied. In addition, polysaccharides isolated were enzymatic hydrolyzed and changes in the monosaccharide composition in the hydrolysis process were studied. The characteristic viscosities of the initial and hydrolyzed galactomannans were 4.85–3.80 dL/g, respectively. Molecular weights of the galactomannans, after treatment with the enzyme, changed in a range of 55 600–26 200 Da. Studies with ¹H and ¹³C NMR spectroscopy methods showed decreases in galactoside content of the hydrolyzed galactomannans, indicating the primary course of hydrolysis occurs by the side chain of the macromolecule. Increase in duration of enzymatic hydrolysis more than 24 h led to the formation of poly-mannans with a minor content of galactose units. The data can serve to improve isolation and preparation of galactomannan based materials for biomedical purposes.

中文翻译：

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植物材料中半乳甘露聚糖的理化性质分离与研究

摘要—

该研究的目的是从植物材料中分离半乳甘露聚糖并研究其物理和化学性质。一般从半乳甘露聚糖中分离和纯化一般由半乳甘露聚糖构成的多糖，所述半乳甘露聚糖来自植物槐花和毛led木种子。通过冷热提取方法。定量表征了半乳甘露聚糖中半乳糖和甘露糖的含量，并研究了多糖的理化性质。另外，分离出的多糖被酶水解，并研究了水解过程中单糖组成的变化。初始和水解半乳甘露聚糖的特征粘度分别为4.85–3.80 dL / g。用该酶处理后，半乳甘露聚糖的分子量在55 600–26 200 Da范围内变化。用¹ H和^13研究13 C NMR光谱法显示水解的半乳甘露聚糖的半乳糖苷含量降低，表明水解的主要过程发生在大分子的侧链上。超过24小时的酶促水解持续时间增加导致形成了半乳糖单元含量较少的聚甘露聚糖。该数据可用于改善基于半乳甘露聚糖的材料的分离和制备，以用于生物医学目的。