When cultivating the kelp Saccharina latissima, knowledge on the availability of seeding material for the production is essential. Applying a spore seeding approach requires spores from the reproductive organs of the fertile sporophytes (sori). As sori are generally not present during the time of seeding in late summer, the production of spores (sporogenesis) can be artificially induced by removing the meristematic part of the sporophyte and keeping the sporophyte under short day, temperate, and nutrient-replete conditions. Only limited information is available on the effect of light intensities including darkness on the sporogenesis of S. latissima. This study examined the natural pattern of sporogenesis in S. latissima from Middelfart, Denmark, and the effect of four different light regimes (0, 20, 60, or 120 μmol photons m⁻² s⁻¹) on the artificial induction of sporogenesis in S. latissima. Natural reproductivity and availability of spores in Denmark peaked in early winter, with 86% of the population being reproductive in November. Reproductive material was available from October until late spring, but with a variable spore release from 11 × 10³ to 1.2 × 10⁶ spores cm⁻² sori. The artificial induction of sporogenesis was optimal in darkness with > 90% of sporophytes developing sori after 49 days, with an average spore release density of 1.15 ± 0.38 × 10⁶ spores cm⁻² sori. The results confirmed that S. latissima in Denmark follows the general pattern of reproduction of S. latissima in North Atlantic regions and demonstrated for the first time that sporogenesis in S. latissima can be efficiently induced in darkness.

在培养海带Saccharina latissima时，必须掌握有关生产用种子材料的知识。应用孢子播种方法需要来自可育孢子体（sori）生殖器官的孢子。由于夏末播种时通常不存在孢子，因此可以通过除去孢子体的分生组织部分并将孢子体保持在短日，温带和营养丰富的条件下来人工诱导孢子的产生（孢子发生）。关于包括黑暗在内的光照强度对S. latissima孢子发生的影响的信息很少。这项研究检查了S. latissima孢子发生的自然模式从米泽尔法特，丹麦，和四个不同的光环境（0，20，60，或120微摩尔光子米效果^-2小号^-1上在人工诱导的孢子发生）S. latissima。丹麦的自然繁殖力和孢子的可获得性在冬季初达到顶峰，11月有86％的人口繁殖。从十月到春季末可获得生殖材料，但孢子释放量从11×10 ³到1.2×10 ⁶孢子cm ^-2 sori不等。孢子发生的人工诱导是最佳的，在黑暗中> 90％的孢子在49天后发育为孢子，平均孢子释放密度为1.15±0.38×10 ⁶孢子cm⁻² sori。结果证实丹麦的S. latissima遵循北大西洋地区S. latissima繁殖的一般模式，并首次证明在黑暗中可有效诱导S. latissima的孢子发生。