Land use alteration such as livestock grazing can affect water quality in habitats of at-risk wildlife species. Data from managed wetlands are needed to understand levels of exposure for aquatic life stages and monitor grazing-related changes afield. We quantified spatial and temporal variation in water quality in wetlands occupied by threatened Oregon spotted frog (Rana pretiosa) at Klamath Marsh National Wildlife Refuge in Oregon, United States (US). We used analyses for censored data to evaluate the importance of habitat type and grazing history in predicting concentrations of nutrients, turbidity, fecal indicator bacteria (FIB; total coliforms, Escherichia coli (E. coli), and enterococci), and estrogenicity, an indicator of estrogenic activity. Nutrients (orthophosphate and ammonia) and enterococci varied over time and space, while E. coli, total coliforms, turbidity, and estrogenicity were more strongly associated with local livestock grazing metrics. Turbidity was correlated with several grazing-related constituents and may be particularly useful for monitoring water quality in landscapes with livestock use. Concentrations of orthophosphate and estrogenicity were elevated at several sites relative to published health benchmarks, and their potential effects on Rana pretiosa warrant further investigation. Our data provided an initial assessment of potential exposure of amphibians to grazing-related constituents in western US wetlands. Increased monitoring of surface water quality and amphibian population status in combination with controlled laboratory toxicity studies could help inform future research and targeted management strategies for wetlands with both grazing and amphibians of conservation concern.

土地用途的改变（例如放牧牲畜）会影响处于危险中的野生动植物物种栖息地的水质。需要管理湿地的数据来了解水生生命阶段的暴露水平，并监测野外与放牧相关的变化。我们对美国俄勒冈州克拉马斯沼泽国家野生动物保护区受威胁的俄勒冈斑蛙（Rana pretiosa）占用的湿地中水质的时空变化进行了量化。我们使用分析数据进行审查，以评估栖息地类型和放牧历史在预测养分，浑浊度，粪便指示细菌（FIB；总大肠菌群，大肠杆菌（E. coli）），肠球菌）和雌激素性（雌激素活性的指标）。营养物质（正磷酸盐和氨水）和肠球菌随时间和空间而变化，而大肠杆菌，总大肠菌群，浊度和雌激素性与当地牲畜放牧指标更紧密相关。浊度与几种与放牧有关的成分有关，对于监测牲畜使用景观的水质可能特别有用。相对于已公布的健康基准，几个地方的正磷酸盐和雌激素浓度都升高了，并且它们对珍稀林蛙的潜在影响需要进一步调查。我们的数据提供了对美国西部湿地两栖动物与放牧相关成分的潜在暴露的初步评估。加强对地表水水质和两栖动物种群状况的监测，再加上受控的实验室毒性研究，可能有助于为有放牧和两栖动物保护需求的湿地提供进一步的研究和针对性的管理策略。