The study aims at investigating the growth and development of two common pests in mulberry namely the leaf roller (Diaphania pulverulentalis) and mealy bug (Macconellicoccus hirsutus), reared on transgenic mulberry plants in comparison with the wild type plants V1, a ruling variety. Both the pests completed normal life cycle on all the four different transgenic plants (Ip::HVA1, Cp::HVA1, Cp::BCH1, Cp::osmotin and Ip::osmotin) expressing three different transgenes (HVA1, BCH1 and osmotin) in the presence of the marker gene NPTII. There was no significant difference in the incubation period of the eggs, growth of first to fifth instar larvae and total larval period of the leaf roller reared on transgenic and non-transgenic mulberry. The pre-pupal, pupal, adult stages and adult fecundity also did not differ. The variations in the duration of the different nymphal instars, and their total duration was not significant in the case of mealy bug, when reared on the transgenics. The adult longevity and total life span of female mealy bugs, and the pupal period and total life span of the male bugs were on par with those reared on the wild type plants. The study indicates that the life cycle of both the pests, which are common in a mulberry ecosystem, were not affected by feeding on any of the transgenic mulberry plants at any stage of their growth and development.

该研究旨在调查桑树中两种常见害虫的生长和发育，即在转基因桑树上饲养的卷叶虫 ( Diaphania pulverulentalis ) 和粉蚧 ( Macconellicoccus hirsutus )，与野生型植物 V1（一种主要品种）进行比较。这两种害虫在所有四种不同的转基因植物（Ip::HVA1、Cp::HVA1、Cp::BCH1、Cp:: osmotin和 Ip:: osmotin）上都完成了正常的生命周期，表达了三种不同的转基因（HVA1、BCH1和osmotin ） ) 在标记基因NPTII存在的情况下. 转基因和非转基因桑树的卵孵化期、1-5龄幼虫生长和卷叶轮总幼虫期无显着差异。前蛹、蛹、成虫阶段和成虫繁殖力也没有差异。在转基因饲养的粉蚧的情况下，不同若虫龄期的持续时间及其总持续时间的变化不显着。雌粉蝽的成虫寿命和总寿命，雄虫的蛹期和总寿命与野生型植物饲养的相当。研究表明，这两种害虫在桑树生态系统中很常见，它们的生命周期不受任何转基因桑树生长发育阶段的摄食的影响。