Allyl triphenylphosphonium bromide based mito-tracker capped silicon quantum dot (Mito-SiQDs) has been synthesized through an inverse micelle process. It was then fully characterized by transmission electron microscopy, energy-dispersive X-ray spectroscopy, dynamic light scattering techniques and X-ray photoelectron spectroscopic method. Energy dispersive X-ray spectroscopy analyses of the quantum dots confirm the presence of carbon, silicon, phosphorous and bromine atoms in Mito-SiQDs. Morphological study by transmission electron microscopy experiment showed the formation of the particles of size 11-12 nm of quantum dot dimension. The high negative zeta potential value of –23.7 mV calculated from dynamic light scattering study indicates the high stability of the circumvent agglomeration of Mito-SiQDs. The mito-tracker capped silicon quantum dot has blue emission at 400 nm wavelength upon excitation at 327 nm. Mito-SiQDs has not shown any significant cytotoxic effect with 10 to 50 μL/mL concentration on HeLa cell line for at least up to 12 h of its treatment. The Mito-SiQDs would be useful a possible fluorescent marker to visualize mitochondrial subcellular compartment in living cell through fluorescence imaging study.

中文翻译：

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新型水溶性，无细胞毒性的微型示踪剂封端的硅量子点的合成与表征

通过反胶束法合成了基于烯丙基三苯基溴化mi的线粒体跟踪的硅量子点（Mito-SiQDs）。然后通过透射电子显微镜，能量色散X射线光谱，动态光散射技术和X射线光电子光谱法对其进行了全面表征。量子点的能量色散X射线光谱分析证实了Mito-SiQD中存在碳，硅，磷和溴原子。通过透射电子显微镜实验的形态学研究表明形成了量子点尺寸为11-12nm的颗粒。根据动态光散射研究计算得出的–23.7 mV的高负zeta电位值表明Mito-SiQD规避聚集的高度稳定性。在327 nm处激发时，覆盖有米托-跟踪器的硅量子点在400 nm波长处具有蓝色发射。Mito-SiQD在至少12 h的治疗中对HeLa细胞系的浓度为10至50μL/ mL时未显示任何明显的细胞毒性作用。该美图- SiQDs通过荧光成像研究，可能有用的荧光标记物在活细胞中可视化线粒体亚细胞区室。