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Highly selective enrichment and direct determination of imazethapyr residues from milk using magnetic solid-phase extraction based on restricted-access molecularly imprinted polymers
Analytical Methods ( IF 3.1 ) Pub Date : 2020-12-29 , DOI: 10.1039/d0ay02116d
Shicong Jia 1, 2, 3, 4, 5 , Yanqiang Zhou 1, 2, 3, 4, 5 , Jianmin Li 1, 2, 3, 4, 5 , Bolin Gong 1, 2, 3, 4, 5 , Shujuan Ma 6, 7, 8, 9, 10 , Junjie Ou 6, 7, 8, 9, 10
Affiliation  

Restricted access media magnetic molecularly imprinted polymers (RAM-MMIPs) were prepared as magnetic solid phase extraction (M-SPE) material by reversible addition fragmentation chain transfer (RAFT) technique. The resulting RAM-MMIPs had a uniform, imprinted, hydrophilic layer (63 nm), good binding capacity (34.85 mg g−1) and satisfactory selectivity. In addition, these RAM-MMIPs had a robust ability to eliminate the interference of protein macromolecules. These RAM-MMIPs were then coupled with HPLC/UV to identify imazethapyr (IM) residues in untreated milk samples. Several major factors would affect M-SPE extraction efficiency, such as the amount of RAM-MMIPs, pH, extraction time of the sample solution, and the volume ratio of the elution solvent. Under the optimal conditions, the developed method had good linearity (R2 > 0.9993), low detection limit (2.13 μg L−1), and low quantitative limit (7.15 μg L−1). These results indicated this proposed approach is an efficient method for direct enrichment and detection of IM herbicides in milk and other biological samples.

中文翻译:

基于受限访问的分子印迹聚合物的磁性固相萃取对牛奶中的咪唑乙烟碱残留进行高选择性富集和直接测定

通过可逆加成断裂链转移(RAFT)技术将限制性访问介质磁性分子印迹聚合物(RAM-MMIPs)制备为磁性固相萃取(M-SPE)材料。所得的RAM-MMIP具有均匀的印迹亲水层(63 nm),良好的结合能力(34.85 mg g -1)和令人满意的选择性。此外,这些RAM-MMIP具有消除蛋白质大分子干扰的强大能力。然后将这些RAM-MMIP与HPLC / UV结合以鉴定未经处理的牛奶样品中的咪唑乙烟(IM)残留物。几个主要因素会影响M-SPE的萃取效率,例如RAM-MMIPs的量,pH,样品溶液的萃取时间以及洗脱溶剂的体积比。在最佳条件下,所开发的方法具有良好的线性(R 2 > 0.9993),低检测限(2.13μgL -1)和低定量限(7.15μgL -1)。)。这些结果表明,该提议的方法是一种直接富集和检测牛奶和其他生物样品中IM除草剂的有效方法。
更新日期:2021-01-12
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