The stressed cultivations are widely used in microalgae R&D for the biofuel production with the repress on growth to a certain degree, which limits the overall productivity. The balance between the growth and energy storage compounds accumulation is a target needing the combination of both strain selection or construction and culture optimization. Here, an engineered strain of Chlamydomonas reinhardtii, in which the chloroplast type glyceraldehyde-3-phosphate dehydrogenase (cGAPDH) was overexpressed and named as P3-GAPDH, was cultured on the Algal Station platform. Compared with wild type (WT), C. reinhardtii CC137c, in Tris-acetate-phosphate (TAP) medium, the highest density of WT and P3-GAPDH were 1.23 ± 0.13 and 1.74 ± 0.09 g L–1 within 96 h, and the maximum biomass productivity was 24.30 ± 1.65 and 28.54 ± 1.43 mg L–1 h–1, respectively. In terms of the energy storage compounds, both carbohydrate and fatty acids content doubled in P3-GAPDH, from 0.13 ± 0.02 to 0.26 ± 0.04 g L–1 for carbohydrate and from 0.08 ± 0.01 to 0.16 ± 0.01 g L–1 for fatty acids, among which poly unsaturated fatty acids increased by 65.8%. Together with the continuous monitor of the chlorophyll fluorescence dynamics parameters Fv/Fm and Fv’/Fm’ and pH of culture, enhanced Calvin cycle by overexpressed cGAPDH promoted the carbon conversion and subsequent energy storage compounds accumulation. C. reinhardtii P3-GAPDH strain showed the potential as a good chassis with high carbon conversion ability.

中文翻译：

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在非压力条件下实现生长和生物燃料生产双赢的碳固定增强莱茵衣藻菌株

胁迫栽培广泛用于生物燃料生产的微藻研发，在一定程度上抑制了生长，限制了整体生产力。生长和储能化合物积累之间的平衡是需要菌株选择或构建和培养优化相结合的目标。在这里，在藻站平台上培养了一种莱茵衣藻工程菌株，其中叶绿体型 3-磷酸甘油醛脱氢酶 (cGAPDH) 过表达并命名为 P3-GAPDH。与野生型（WT）莱茵衣藻 CC137c 相比，在 Tris-醋酸-磷酸盐（TAP）培养基中，WT 和 P3-GAPDH 的最高密度在 96 小时内分别为 1.23 ± 0.13 和 1.74 ± 0.09 g L-1，并且最大生物量生产力分别为 24.30 ± 1.65 和 28.54 ± 1.43 mg L–1 h–1。在储能化合物方面，P3-GAPDH 中碳水化合物和脂肪酸的含量都翻了一番，碳水化合物从 0.13 ± 0.02 增加到 0.26 ± 0.04 g L–1，脂肪酸从 0.08 ± 0.01 增加到 0.16 ± 0.01 g L–1 ，其中多不饱和脂肪酸增加65.8%。连同对叶绿素荧光动力学参数 Fv/Fm 和 Fv'/Fm' 以及培养物 pH 值的连续监测，过表达的 cGAPDH 增强了卡尔文循环，促进了碳转化和随后的储能化合物积累。C. reinhardtii P3-GAPDH 菌株显示出作为具有高碳转化能力的良好底盘的潜力。其中多不饱和脂肪酸增加65.8%。连同对叶绿素荧光动力学参数 Fv/Fm 和 Fv'/Fm' 以及培养物 pH 值的连续监测，过表达的 cGAPDH 增强了卡尔文循环，促进了碳转化和随后的储能化合物积累。C. reinhardtii P3-GAPDH 菌株显示出作为具有高碳转化能力的良好底盘的潜力。其中多不饱和脂肪酸增加65.8%。连同对叶绿素荧光动力学参数 Fv/Fm 和 Fv'/Fm' 以及培养物 pH 值的连续监测，过表达的 cGAPDH 增强了卡尔文循环，促进了碳转化和随后的储能化合物积累。C. reinhardtii P3-GAPDH 菌株显示出作为具有高碳转化能力的良好底盘的潜力。