Cardiovascular disease (CVD) remains the leading cause of morbidity and mortality around the world. Early diagnosis of CVD could provide the opportunity for sensible management and better clinical outcome along with the prevention of further progression of the disease. In the current study, we used an untargeted metabolomic approach to identify possible metabolite(s) that associate well with the CVD and could serve either as therapeutic target or disease-associated metabolite. We identified 26 rationally adjusted unique metabolites that were differentially present in the serum of CVD patients compared with healthy individuals, among them 15 were found to be statistically significant. Out of these metabolites, we identified some novel metabolites like UDP- l -rhamnose and N 1-acetylspermidine that have not been reported to be linked with CVD directly. Further, we also found that some metabolites like ethanolamide, solanidine, dimethylarginine, N -acetyl- l -tyrosine, can act as a discriminator of CVD. Metabolites integrating pathway enrichment analysis showed enrichment of various important metabolic pathways like histidine metabolism, methyl histidine metabolism, carnitine synthesis, along with arginine and proline metabolism in CVD patients. Our study provides a great opportunity to understand the pathophysiological role and impact of the identified unique metabolites and can be extrapolated as specific CVD specific metabolites.

中文翻译：

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使用非靶向代谢组学鉴定新的心血管疾病相关代谢物

心血管疾病 (CVD) 仍然是全球发病率和死亡率的主要原因。CVD 的早期诊断可以提供明智的管理和更好的临床结果以及预防疾病进一步进展的机会。在目前的研究中，我们使用非靶向代谢组学方法来确定可能与 CVD 相关联的代谢物，并可作为治疗靶点或疾病相关代谢物。我们确定了 26 种经过合理调整的独特代谢物，与健康个体相比，它们在 CVD 患者的血清中存在差异，其中 15 种被发现具有统计学意义。在这些代谢物中，我们确定了一些新的代谢物，如 UDP-1-鼠李糖和 N 1-乙酰亚精胺，这些代谢物尚未被报道与 CVD 直接相关。此外，我们还发现一些代谢物，如乙醇酰胺、茄碱、二甲基精氨酸、N-乙酰基-l-酪氨酸，可以作为 CVD 的鉴别器。代谢物整合通路富集分析显示，心血管疾病患者的组氨酸代谢、甲基组氨酸代谢、肉碱合成以及精氨酸和脯氨酸代谢等各种重要代谢通路均富集。我们的研究提供了一个很好的机会来了解已鉴定的独特代谢物的病理生理作用和影响，并且可以推断为特定的 CVD 特定代谢物。可以作为 CVD 的鉴别器。代谢物整合通路富集分析显示，心血管疾病患者的组氨酸代谢、甲基组氨酸代谢、肉碱合成以及精氨酸和脯氨酸代谢等各种重要代谢通路均富集。我们的研究提供了一个很好的机会来了解已鉴定的独特代谢物的病理生理作用和影响，并且可以推断为特定的 CVD 特定代谢物。可以作为 CVD 的鉴别器。代谢物整合通路富集分析显示，心血管疾病患者的组氨酸代谢、甲基组氨酸代谢、肉碱合成以及精氨酸和脯氨酸代谢等各种重要代谢通路均富集。我们的研究提供了一个很好的机会来了解已鉴定的独特代谢物的病理生理作用和影响，并且可以推断为特定的 CVD 特定代谢物。