Meiosis is a specialized cell division process that mediates genetic information transfer to the next generation. Meiotic chromosomal segregation occurs when DNA replication is completed during the pre-meiotic S phase. Here, we show that Schizosaccharomyces pombe Pef1, an orthologue of mammalian cyclin-dependent kinase 5 (CDK5), is required to promote pre-meiotic DNA replication. We examined the efficiency of meiotic initiation using pat1-114 mutants and found that, meiotic nuclear divisions did not occur in the pef1Δ pat1-114 strain. Deletion of pef1 also suppressed the expression of DNA replication factors and the phosphorylation of Cdc2 Tyr-15. The double deletion of clg1 and psl1 arrested meiotic initiation in pat1-114 mutant cells, similar to that of pef1-deficient cells. Meiotic progression was also slightly delayed in the pas1-deficient strain. Our results reveal that Pef1 regulates cyclin-coordinated meiotic progression.

中文翻译：

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粟酒裂殖酵母CDK5直系同源蛋白Pef1与三个细胞周期蛋白Clg1，Pas1和Psl1协同促进促减数分裂前DNA复制

减数分裂是一种专门的细胞分裂过程，介导遗传信息传递给下一代。当减数分裂前S期DNA复制完成时，发生减数分裂染色体分离。在这里，我们显示粟酒裂殖酵母Pef1，哺乳动物细胞周期蛋白依赖性激酶5（CDK5）的直系同源，是促进减数分裂前DNA复制所必需的。我们检查了使用pat1-114突变体进行减数分裂启动的效率，发现在pef1Δpat1-114菌株中未发生减数分裂核分裂。pef1的删除也抑制了DNA复制因子的表达和Cdc2 Tyr-15的磷酸化。clg1和psl1的双重删除在pat1-114突变细胞中阻止了减数分裂起始，类似于缺乏pef1的细胞。在pas1缺陷型菌株中，减数分裂进程也略有延迟。我们的结果表明，Pef1调节细胞周期蛋白协调的减数分裂进程。