Hypertrophic and hypoxic visceral adipose tissue (VAT) secretes proinflammatory cytokines promoting insulin resistance (IR), prediabetes and type 2 diabetes (T2DM) microRNAs (miRNAs) are markers of metabolic disorders regulating genes critical for e.g., inflammation, glucose metabolism, and antioxidant defense, with raising diagnostic value. The aim of the current study was to evaluate whether hyperglycemia is able to affect the expression of selected miRNAs in VAT of prediabetic (IFG) and diabetic (T2DM) patients vs. normoglycemic (NG) subjects using qPCR. Statistical analyses suggested that miRNAs expression could be sex-dependent. Thus, we determined 15 miRNAs as differentially expressed (DE) among NG, T2DM, IFG females (miR-10a-5p, let-7d-5p, miR-532-5p, miR-127-3p, miR-125b-5p, let-7a-5p, let-7e-5p, miR-199a-3p, miR-365a-3p, miR-99a-5p, miR-100-5p, miR-342-3p, miR-146b-5p, miR-204-5p, miR-409-3p). Majority of significantly changed miRNAs was similarly upregulated in VAT of female T2DM and IFG patients in comparison to NG subjects, positively correlated with FPG and HbA1c, yet, uncorrelated with WHR/BMI. Enrichment analyses indicated involvement of 11 top DE miRNAs in oxidative stress, inflammation and insulin signaling. Those miRNAs expression changes could be possibly associated with low-grade chronic inflammation and oxidative stress in VAT of hyperglycemic subjects.

中文翻译：

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糖尿病前期女性和糖尿病女性的内脏脂肪组织共有一组类似的上调的microRNA，功能上被标注为炎症，氧化应激和胰岛素信号传导

肥厚和缺氧的内脏脂肪组织（VAT）分泌促胰岛素抵抗（IR），糖尿病前期和2型糖尿病（T2DM）的促炎细胞因子microRNA（miRNA）是代谢紊乱的标志物，其调节对炎症，葡萄糖代谢和抗氧化防御至关重要的基因，具有提高诊断价值。本研究的目的是使用qPCR评估高血糖症是否能够影响糖尿病前期（IFG）和糖尿病（T2DM）患者与正常血糖（NG）患者的增值税中所选miRNA的表达。统计分析表明，miRNA的表达可能是性别依赖性的。因此，我们确定了NG，T2DM，IFG雌性（miR-10a-5p，let-7d-5p，miR-532-5p，miR-127-3p，miR-125b-5p， let-7a-5p，let-7e-5p，miR-199a-3p，miR-365a-3p，miR-99a-5p，miR-100-5p，miR-342-3p，miR-146b-5p，miR-204-5p，miR-409-3p）。与NG受试者相比，女性T2DM和IFG患者的增值税中，显着变化的miRNA的大多数也上调，与FPG和HbA1c正相关，但与WHR / BMI不相关。富集分析表明11种顶级DE miRNA参与了氧化应激，炎症和胰岛素信号传导。这些miRNA表达变化可能与高血糖受试者的增值税中的低度慢性炎症和氧化应激有关。富集分析表明11种顶级DE miRNA参与了氧化应激，炎症和胰岛素信号传导。这些miRNA表达变化可能与高血糖受试者的增值税中的低度慢性炎症和氧化应激有关。富集分析表明11种顶级DE miRNA参与了氧化应激，炎症和胰岛素信号传导。这些miRNA表达变化可能与高血糖受试者的增值税中的低度慢性炎症和氧化应激有关。