This study was conducted to evaluate the effect of Haematococcus pluvialis powder on astaxanthin content and isomer composition, antioxidant ability and immunity of Litopenaeus vannamei after short‐term feedings. Experimental diets include a commercial diet (control) and diets containing H. pluvialis powder (HP‐35 and HP‐70, 35 and 70 mg of carotenoids kg⁻¹). After feeding for 15 days, the content of astaxanthin in the muscle, cephalothorax and shell of shrimp increased significantly with increasing addition of H. pluvialis powder (p < 0.05). Dietary addition of H. pluvialis powder significantly increased the activity of total antioxygenic capacity (T‐AOC), superoxide dismutase (SOD) and glutathione S‐transferase (GST) as well as the relative mRNA levels of immune‐related genes, Toll, MyD88, IMD, Crustin, PO and Lysozyme in hepatopancreas (p < 0.05). The proportion of astaxanthin isomers in different body parts was different, whereas it has no significant difference between same body parts of different groups. This indicated that the accumulation of astaxanthin among body parts may be accompanied with selection and isomerization. In conclusion, feeding with H. pluvialis powder diets containing 35 and 70 mg of carotenoids kg⁻¹ can enhance the carotenoid and astaxanthin content, antioxidant and immune capacity of L. vannamei.

中文翻译：

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补充日粮血红球菌粉会影响太平洋白对虾凡纳滨对虾中类胡萝卜素含量，虾青素异构体，抗氧化能力和免疫相关基因的表达

本研究旨在评估短期进食后产血红球菌粉对虾青素含量和异构体组成，凡纳滨对虾的抗氧化能力和免疫力的影响。实验饮食包括商业饮食（对照）和含幽门螺杆菌粉的饮食（HP-35和HP-70、35和70 mg类胡萝卜素kg ^-1）。喂食15天后，虾中肌肉，头胸部和壳中虾青素的含量随着H的添加而显着增加。 尿液粉（p  <0.05）。日粮添加^ h。 湿润的粉末显着提高了总抗氧能力（T‐AOC），超氧化物歧化酶（SOD）和谷胱甘肽S-转移酶（GST）的活性，以及​​免疫相关基因的相对mRNA水平，Toll，MyD88，IMD，Crustin，PO和肝胰脏中的溶菌酶（p  <0.05）。虾青素异构体在不同身体部位的比例不同，而在不同群体的相同身体部位之间没有显着差异。这表明虾青素在人体各部位的积累可能伴随着选择和异构化。总之，用H喂食。 含35和70毫克类胡萝卜素kg ^-1的鼠尾草粉饮食可以增强L的类胡萝卜素和虾青素含量，抗氧化和免疫能力。 南美白对虾。