This study was conducted to evaluate the supplemental effects of esterified monobutyrin (MB) and tributyrin (TB) on growth performance, feed efficiency, digestibility, innate immunity, antioxidant status and disease resistance of Pacific white shrimp, Litopenaeus vannamei. A basal (control) experimental diet was formulated without MB or TB, and four other diets were prepared by supplementing powder forms of MB at 2 and 4 g/kg (0.2% and 0.4%) or TB at 1 and 2 g/kg (0.1% and 0.2%) (designated as MB0.2, MB0.4, TB0.1 and TB0.2, respectively). Quadruplicate groups of shrimps were hand‐fed with one of the diets four times daily for 53 days. Shrimp fed MB or TB diets showed significantly higher growth performance, feed utilization, phagocytosis and glutathione peroxidase activity than shrimp fed the control diet. Increased digestibility of dietary protein, lipid and dry matter was found in shrimp fed MB‐ or TB‐supplemented diets. Relative mRNA expression levels of IGF‐binding protein and prophenoloxidase gene were significantly increased in shrimp fed MB‐ or TB‐supplemented diets than in shrimp fed the control diet. The results of a challenge test with Vibrio harveyi showed that the disease resistance of shrimp can be significantly enhanced against V. harveyi when they were fed TB0.2 diet. The results of this study indicate that dietary supplementation of 2 g/kg MB or 1–2 g/kg TB can improve growth and feed efficiency, innate immunity, antioxidant activity, digestibility and disease resistance of L. vannamei.

中文翻译：

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日粮中添加单丁酸和三丁酸甘油酯对南美白对虾哈维氏弧菌生长，饲料效率，先天免疫力，消化率和抗病性的影响

这项研究旨在评估酯化单丁酸（MB）和三丁酸甘油酯（TB）对太平洋白对虾凡纳滨对虾的生长性能，饲料效率，消化率，先天免疫力，抗氧化剂状态和抗病性的补充作用。。配制了不含MB或TB的基础（对照）实验饮食，并通过补充2和4 g / kg（0.2％和0.4％）的MB或1和2 g / kg的TB粉末形式制备了其他四种饮食（ 0.1％和0.2％）（分别指定为MB0.2，MB0.4，TB0.1和TB0.2）。每天四次给一组虾饲喂其中一种饮食，共53天。虾饲喂MB或TB日粮比饲喂对照日粮的虾显示出更高的生长性能，饲料利用率，吞噬作用和谷胱甘肽过氧化物酶活性。在以MB或TB补充饲料的虾中，发现日粮中蛋白质，脂质和干物质的消化率增加。饲喂MB或TB的日粮中，IGF结合蛋白和前酚氧化酶基因的相对mRNA表达水平显着高于对照日粮中的虾。哈维弧菌显示，喂食TB0.2日粮时，虾对哈维弧菌的抗病性可以显着增强。这项研究的结果表明，日粮补充2 g / kg MB或1-2 g / kg TB可以改善南美白对虾的生长和饲料效率，先天免疫，抗氧化活性，消化率和抗病性。