Laccase is an effective biocatalyst in bioremediation process; however, the application of the enzyme is limited due to its cost, recovery, and stability. In this study, we developed, characterized and evaluated the efficiency of immobilized laccase on zinc oxide nanostructure to catalyze biodegradation of TBA in comparison to the suspended enzyme. The results showed that both immobilized and suspended laccase were capable of catalyzing TBA biodegradation; however, the efficiency of the immobilized laccase on TBA removal was higher than that of the suspended enzyme. The repeatability testing revealed the potential of the immobilized laccase for repeatedly catalyzing TBA biodegradation with storage capacity. While the V_max of immobilized enzyme was higher than suspended laccase (2.25 ± 0.542 mg TBA/h∙U vs. 1.47 ± 0.185 mg TBA/h∙U), the k_m of the immobilized enzyme was higher than the suspended laccase (67.9 ± 20.5 mg TBA/L vs. 33.5 ± 7.10 mg TBA/L). This suggests that the immobilized laccase is better in TBA removal, but has lower affinity with TBA than the suspended enzyme. Thus, immobilization of the enzyme can be applied to increase the efficiency and minimize the use of laccase for TBA remediation.

漆酶是生物修复过程中的有效生物催化剂。然而，由于其成本，回收率和稳定性，该酶的应用受到限制。在这项研究中，我们开发，表征和评估了固定化漆酶在氧化锌纳米结构上与悬浮酶相比催化TBA的生物降解的效率。结果表明，固定和悬浮漆酶均能催化TBA的生物降解。然而，固定的漆酶去除TBA的效率高于悬浮酶。重复性测试揭示了固定化漆酶具有重复催化TBA生物降解和储存能力的潜力。而V _max固定化酶的酶活力高于悬浮漆酶（2.25±0.542 mg TBA / h∙U对比1.47±0.185 mg TBA / h∙U），固定酶的k _m高于悬浮漆酶（67.9±20.5 mg TBA / L vs.33.5±7.10 mg TBA / L）。这表明固定化的漆酶具有更好的TBA去除能力，但与TBA的亲和力比悬浮酶低。因此，可以应用酶的固定化以提高效率并最小化漆酶用于TBA修复。