The aim of this study was to investigate the changes in and clinical significance of circular RNAs (circRNAs) expression related to immunity and inflammation in patients with rheumatoid arthritis (RA). We analyzed the circRNAs related to immunity and inflammation in peripheral blood mononuclear cells (PBMCs) from three RA patients and three healthy controls using high-throughput sequencing and bioinformatics analysis. Subsequently, four differentially expressed circRNAs were verified by reverse transcriptase quantitative polymerase chain reaction (RT-qPCR). We selected hsa_circ_0003353 for functional phenotype analysis in fibroblast-like synoviocytes (FLS) according to correlation tests and logistic regression analysis. The results identified and verified the expression of circRNAs (0003353, 0005732, 0072428, 0091685) that were significantly changed in PBMCs from RA patients. ROC analysis suggested that these circRNAs have significant value in RA diagnosis. Spearman analysis revealed that significant relationships between these circRNAs and clinical indicators as well as SPP. The logistic regression analysis showed that clinical indicators and SPP were risk factors for hsa_circ_0003353 expression, which is worthy of further investigation. As predicted, hsa_circ_0003353 had significantly higher expression in RA-FLS. The cell proliferation, migration, invasion, apoptosis, and inflammatory cytokine production of RA-FLS showed significant alterations after hsa_circ_0003353 suppression and overexpression. Our results indicate that hsa_circ_0003353 may play an essential role in promoting immunity, inflammation, synovial invasion, and joint destruction and that hsa_circ_0003353 may act as a biomarker of RA.

这项研究的目的是调查与类风湿关节炎（RA）患者的免疫力和炎症相关的环状RNA（circRNA）表达的变化及其临床意义。我们使用高通量测序和生物信息学分析方法，对来自三名RA患者和三名健康对照者的外周血单核细胞（PBMC）中与免疫和炎症相关的circRNA进行了分析。随后，通过逆转录酶定量聚合酶链反应（RT-qPCR）验证了四个差异表达的circRNA。根据相关性测试和逻辑回归分析，我们选择hsa_circ_0003353在成纤维样滑膜细胞（FLS）中进行功能表型分析。结果鉴定并验证了circRNA（0003353、0005732、0072428，0091685）在RA患者的PBMC中发生了显着变化。ROC分析表明，这些circRNA在RA诊断中具有重要价值。Spearman分析表明，这些circRNA与临床指标以及SPP之间存在显着关系。Logistic回归分析表明，临床指标和SPP是hsa_circ_0003353表达的危险因素，值得进一步研究。如所预测的，hsa_circ_0003353在RA-FLS中具有明显更高的表达。在hsa_circ_0003353抑制和过表达后，RA-FLS的细胞增殖，迁移，侵袭，凋亡和炎性细胞因子产生显示出明显的变化。我们的结果表明，hsa_circ_0003353可能在促进免疫力，炎症，滑膜浸润，